CAPAM/PCIF1 (E8B1B) Rabbit Monoclonal Antibody#59921,Cell Signaling Technology (CST),59921

CAPAM/PCIF1 (E8B1B) Rabbit Monoclonal Antibody recognizes endogenous levels of total CAPAM/PCIF1 protein.

Host

Rabbit

Reactivity

Human, Mouse, Rat, Monkey

Application

Western Blotting: 1:1000 Immunoprecipitation: 1:100

Platform ID

BAB029918719

Cell Signaling Technology (CST)

Headquarters

3 Trask Lane Danvers, MA 01923

Cell Signaling Technology (CST)

Contact

Tel: 877-616-2355,978-867-2388
Fax: 877-616-2355

Email:

[email protected]

Product Specifications
Scientific Background
Synonyms

Specifications

NameCAPAM/PCIF1 (E8B1B) Rabbit Monoclonal Antibody#59921
Cat. No.59921
Accession NumberQ9H4Z3
Gene ID (Entrez)63935
HostRabbit
SensitivityEndogenous
ReactivityHuman, Mouse, Rat, Monkey
ApplicationWestern Blotting: 1:1000 Immunoprecipitation: 1:100
Molecular Weight85
ImmunogenIgG
FormulationSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C.Do not aliquot the antibody.
StorageSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C.Do not aliquot the antibody.
Regulatory StatusResearch Use Only

Scientific Background

Phosphorylated CTD Interacting Factor 1 (PCIF1), also known as CAPAM, is a WW domain-containing protein that was initially discovered to interact with phosphorylated RNA Polymerase II, where it inhibits CTD phosphatase activity of SCP1 to negatively affect gene expression (1,2). CAPAM/PCIF1 has shown to be the methyltransferase responsible for methylating adenosine at the second position of mRNAs, promoting their translation (3,4).Fan, H. et al. (2003)Biochem Biophys Res Commun301, 378-85.Hirose, Y. et al. (2008)Biochem Biophys Res Commun369, 449-55.Akichika, S. et al. (2019)Science363, pii: eaav0080. doi: 10.1126/science.aav0080.Cowling, V.H. (2019)Trends Biochem Sci44, 183-5.Alternate NamesC20orf67; Cap-specific adenosine methyltransferase; CAPAM; hCAPAM; hPCIF1; mRNA (2'-O-methyladenosine-N(6)-)-methyltransferase; PCIF1; PDX-1 C terminus-interacting factor 1; PDX1 C-terminal inhibiting factor 1; phosphorylated CTD interacting factor 1; Phosphorylated CTD-interacting factor 1; PPP1R121; Protein phosphatase 1 regulatory subunit 121; protein phosphatase 1, regulatory subunit 121

Synonyms

C20orf67; Cap-specific adenosine methyltransferase; CAPAM; hCAPAM; hPCIF1; mRNA (2'-O-methyladenosine-N(6)-)-methyltransferase; PCIF1; PDX-1 C terminus-interacting factor 1; PDX1 C-terminal inhibiting factor 1; phosphorylated CTD interacting factor 1; Phosphorylated CTD-interacting factor 1; PPP1R121; Protein phosphatase 1 regulatory subunit 121; protein phosphatase 1, regulatory subunit 121

Category Paths

Request a product

Please provide the required information below so that we can quickly source your products.