CD19 (Intracellular Domain) (D4V4B) Rabbit Monoclonal Antibody (SignalFlexTMAlexa Fluor®555 Conjugate)#10621,Cell Signaling Technology (CST),10621

CD19 (Intracellular Domain) (D4V4B) XP®Rabbit mAb (SignalFlexTM Alexa Fluor®555 Conjugate) recognizes endogenous levels of total CD19 protein.

Host

Rabbit

Reactivity

Human, Mouse, Monkey

Conjugate

SignalFlexTMAlexa Fluor®555 Conjugate

Platform ID

BAB692524424

Cell Signaling Technology (CST)

Headquarters

3 Trask Lane Danvers, MA 01923

Cell Signaling Technology (CST)

Contact

Tel: 877-616-2355,978-867-2388
Fax: 877-616-2355

Email:

[email protected]

Product Specifications
Scientific Background
Synonyms

Specifications

NameCD19 (Intracellular Domain) (D4V4B) Rabbit Monoclonal Antibody (SignalFlexTMAlexa Fluor®555 Conjugate)#10621
Cat. No.10621
Accession NumberP15391
Gene ID (Entrez)15391
HostRabbit
SensitivityEndogenous
ReactivityHuman, Mouse, Monkey
ConjugationSignalFlexTMAlexa Fluor®555 Conjugate
ImmunogenIgG
FormulationSignalFlexTM conjugates are produced using highly validated Cell Signaling Technology®primary antibodies and conjugation methods that have been rigorously tested, ensuring high-quality conjugates and lot-to-lot consistency. These conjugates are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. However, they are not tested on specific assays.Optimal dilutions/concentrations should be determined by the end user. When performing flow cytometry, we recommend using an isotype control conjugate at the same concentration as the antibody conjugate.
StorageSupplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C.Do not aliquot the antibody. Protect from light. Do not freeze.
Regulatory StatusResearch Use Only

Scientific Background

CD19 is a 95 kDa coreceptor which amplifies the signaling cascade in B cells (1). On the B cell surface, CD19 associates with CD21, CD81, and Leu-13 to exert its function. The cytoplasmic tail of CD19 has nine conserved tyrosine residues playing critical roles in CD19 mediated function by coupling signaling molecules to the receptor (1). After B cell receptor or CD19 ligation, Tyr531 and Tyr500 of CD19 are progressively phosphorylated. This phosphorylation enables the coupling of PI3 kinase and Src family tyrosine kinase to CD19 and activates the PI3K and Src signaling pathways (2,3). Coligation of B cell receptor and CD19 also promotes Tyr409 phosphorylation in CD19. The phosphorylation at these sites enables its binding to Vav and mediates elevated intracellular calcium response, as well as the JNK pathway (4,5).Tedder, T.F. et al. (1997)Immunity6, 107-18.Buhl, A.M. and Cambier, J.C. (1999)J Immunol162, 4438-46.Fujimoto, M. et al. (2000)Immunity13, 47-57.O'Rourke, L.M. et al. (1998)Immunity8, 635-45.Sato, S. et al. (1997)Proc Natl Acad Sci U S A94, 13158-62.Alternate NamesB-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; B4; CD19; CD19 antigen; CD19 molecule; CVID3; Differentiation antigen CD19; MGC12802; T-cell surface antigen Leu-12

Synonyms

B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; B4; CD19; CD19 antigen; CD19 molecule; CVID3; Differentiation antigen CD19; MGC12802; T-cell surface antigen Leu-12

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