CD45 (D3F8Q) Rabbit Monoclonal Antibody (Alexa Fluor®488 Conjugate)#59572,Cell Signaling Technology (CST),59572

CD45 (D3F8Q) Rabbit Monoclonal Antibody (Alexa Fluor®488 Conjugate) recognizes endogenous levels of total CD45 protein. This antibody conjugate is predicted to react with both the CD45.1 and CD45.2 alleles.Conjugates of D3F8Q lack sensitivity in CD45 low-expressing cells, including microglia, in fixed-frozen tissues by immunofluorescence.

Host

Rabbit

Reactivity

Mouse

Application

Immunohistochemistry (Paraffin): 1:50 - 1:200 Immunofluorescence (Frozen): 1:200 Immunofluorescence (Immunocytochemistry): 1:800

Platform ID

BAB380717841

Cell Signaling Technology (CST)

Headquarters

3 Trask Lane Danvers, MA 01923

Cell Signaling Technology (CST)

Contact

Tel: 877-616-2355,978-867-2388
Fax: 877-616-2355

Email:

[email protected]

Product Specifications
Scientific Background
Synonyms

Specifications

NameCD45 (D3F8Q) Rabbit Monoclonal Antibody (Alexa Fluor®488 Conjugate)#59572
Cat. No.59572
Accession NumberP06800
Gene ID (Entrez)06800, 19264
HostRabbit
SensitivityEndogenous
ReactivityMouse
ApplicationImmunohistochemistry (Paraffin): 1:50 - 1:200 Immunofluorescence (Frozen): 1:200 Immunofluorescence (Immunocytochemistry): 1:800
ImmunogenIgG
FormulationSupplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
StorageSupplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
Regulatory StatusResearch Use Only

Scientific Background

The protein phosphatase (PTP) receptor CD45 is a type I transmembrane protein comprised of a pair of intracellular tyrosine phosphatase domains and a variable extracellular domain generated by alternative splicing (1). The catalytic activity of CD45 is a function of the first phosphatase domain (D1) while the second phosphatase domain (D2) may interact with and stabilize the first domain, or recruit/bind substrates (2,3). CD45 interacts directly with antigen receptor complex proteins or activates Src family kinases involved in the regulation of T- and B-cell antigen receptor signaling (1). Specifically, CD45 dephosphorylates Src-family kinases Lck and Fyn at their conserved negative regulatory carboxy-terminal tyrosine residues and upregulates kinase activity. Conversely, studies indicate that CD45 can also inhibit Lck and Fyn by dephosphorylating their positive regulatory autophosphorylation site. CD45 appears to be both a positive and a negative regulator that conducts signals depending on specific stimuli and cell type (1). Human leukocytes including lymphocytes, eosinophils, monocytes, basophils, and neutrophils express CD45, while erythrocytes and platelets are negative for CD45 expression (4).Huntington, N.D. and Tarlinton, D.M. (2004)Immunol Lett94, 167-74.Felberg, J. and Johnson, P. (2000)Biochem Biophys Res Commun271, 292-8.Kashio, N. et al. (1998)J Biol Chem273, 33856-63.Wang, Y. and Johnson, P. (2005)J Biol Chem280, 14318-24.Alternate NamesB220; Cd45; CD45R; L-CA; Leukocyte common antigen; loc; Ly-; Ly-5; Lymphocyte antigen 5; lymphocyte common antigen; Lyt-; Lyt-4; protein tyrosine phosphatase, receptor type, C; Ptprc; Receptor-type tyrosine-protein phosphatase C; T200

Synonyms

B220; Cd45; CD45R; L-CA; Leukocyte common antigen; loc; Ly-; Ly-5; Lymphocyte antigen 5; lymphocyte common antigen; Lyt-; Lyt-4; protein tyrosine phosphatase, receptor type, C; Ptprc; Receptor-type tyrosine-protein phosphatase C; T200

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