CREST (E3P4F) Rabbit Monoclonal Antibody#81662,Cell Signaling Technology (CST),81662

CREST (E3P4F) Rabbit Monoclonal Antibody recognizes endogenous levels of total CREST protein. This antibody does not cross-react with SS18 protein.

Host

Rabbit

Reactivity

Human, Monkey

Application

Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:1600 - 1:6400 Chromatin IP: 1:50

Platform ID

BAB597247650

Cell Signaling Technology (CST)

Headquarters

3 Trask Lane Danvers, MA 01923

Cell Signaling Technology (CST)

Contact

Tel: 877-616-2355,978-867-2388
Fax: 877-616-2355

Email:

[email protected]

Product Specifications
Scientific Background
Synonyms

Specifications

NameCREST (E3P4F) Rabbit Monoclonal Antibody#81662
Cat. No.81662
Accession NumberO75177
Gene ID (Entrez)75177, 26039
HostRabbit
SensitivityEndogenous
ReactivityHuman, Monkey
ApplicationWestern Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:1600 - 1:6400 Chromatin IP: 1:50
Molecular Weight60
ImmunogenIgG
FormulationFor optimal ChIP results, use 10 μL of antibody and 10 μg of chromatin (approximately 4 × 106cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
StorageSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C.Do not aliquot the antibody.
Regulatory StatusResearch Use Only

Scientific Background

Calcium-responsive transactivator (CREST), also known as SS18L1, is a calcium-dependent neuronal BAF family member expressed in the developing brain (1-4). CREST recruits CBP to nuclear bodies in order to regulate cortical and hippocampal dendrite development (1,5). The activity of CREST is aided by the ATPase of the BAF complex, Brg1, which upon calcium influx, will release a phospho-Rb/HDAC1 repressive complex from neuronal genes (4). CREST mutations have been described in ALS. The protein contains a prion-like domain, associates with the ALS protein FUS, forms aggregates, and is included in paraspeckles (6-8). CREST is highly homologous to SS18 and can similarly form fusions with SSX proteins in synovial sarcomas (9,10).Aizawa, H. et al. (2004)Science303, 197-202.Jefferis, G.S. et al. (2004)Science303, 179-81.Middeljans, E. et al. (2012)PLoS One7, e33834.Qiu, Z. and Ghosh, A. (2008)Neuron60, 775-87.Pradhan, A. and Liu, Y. (2004)Neurosci Lett370, 191-5.Chesi, A. et al. (2013)Nat Neurosci16, 851-5.Teyssou, E. et al. (2014)Neurobiol Aging35, 1213.e9-1213.e12.Kukharsky, M.S. et al. (2015)Mol Neurodegener10, 20.Storlazzi, C.T. et al. (2003)Genes Chromosomes Cancer37, 195-200.de Bruijn, D.R. and Geurts van Kessel, A. (2006)Cytogenet Genome Res112, 222-6.Alternate NamesCalcium-responsive transactivator; CREST; KIAA0693; LP2261; MGC26711; MGC78386; SS18-like protein 1; SS18L1; SS18L1 subunit of BAF chromatin remodeling complex; SS18L1, nBAF chromatin remodeling complex subunit; synovial sarcoma translocation gene on chromosome 18-like 1; SYT homolog 1

Synonyms

Calcium-responsive transactivator; CREST; KIAA0693; LP2261; MGC26711; MGC78386; SS18-like protein 1; SS18L1; SS18L1 subunit of BAF chromatin remodeling complex; SS18L1, nBAF chromatin remodeling complex subunit; synovial sarcoma translocation gene on chromosome 18-like 1; SYT homolog 1

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