Caspase 9 (pS196) Antibody,Abbexa,abx031837
Caspase 9 is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspase APAF1; this step is thought to be one of the earliest in the caspase activation cascade.
Host
Rabbit
Reactivity
Human
Application
ELISA, WB, IHC
Conjugate
Unconjugated
Platform ID
BAB064238790
Abbexa
Contact
Tel: +44 (0) 1223 755950
Fax: +44 (0) 1223 755951
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Product Specifications
Specifications
NameCaspase 9 (pS196) Antibody
Cat. No.abx031837
Accession NumberP55211
HostRabbit
IsotypeIgG
ReactivityHuman
ConjugationUnconjugated
ApplicationELISA, WB, IHC
Working DilutionsWB: 1/1000, IHC-P: 1/50 - 1/100. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user.
ClonalityPolyclonal
Molecular WeightCalculated MW: 46.3 kDa
ImmunogenKLH-conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S196 of human caspase 9.
PurityPurified by protein G affinity chromatography. Then, the antibody fraction was peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody was eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Appearance/FormLiquid
FormulationPBS containing 0.09% sodium azide.
StorageAliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
Regulatory StatusResearch Use Only
Category Paths
- Products>Primary Antibodies>IHC Antibodies
- Products>Primary Antibodies>Polyclonal Antibodies
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