Cell Fractionation Antibody Sampler Kit#11843,Cell Signaling Technology (CST),11843

Knowledge of the subcellular location of a protein may reveal the potential role it plays in a variety of cellular processes. Antibodies in the Cell Fractionation Antibody Sampler Kit can be used as a marker to ensure that each subcellular fraction is efficiently separated from the next. MEK1 and MEK2, also called MAPK or Erk Kinases, are dual-specificity protein kinases that function in a mitogen activated protein kinase cascade (1). Apoptosis-inducing factor (AIF) is a ubiquitously expressed flavoprotein that plays a critical role in caspase-independent apoptosis (2). Core histone protein, H3 is one of the primary building blocks of chromatin that makes up the nucleosome (3). Vimentin is a cell-specific intermediate filament with mesenchyme origin that contributes to making up of the cytoskeleton (4). MEK1/2, AIF, histone H3, and vimentin localize to the cytoplasm, mitochondria, nucleus, and cytoskeleton, respectively.Crews, C.M. et al. (1992)Science258, 478-80.Lipton, S.A. and Bossy-Wetzel, E. (2002)Cell111, 147-50.Workman, J.L. and Kingston, R.E. (1998)Annu Rev Biochem67, 545-79.Eng, L.F. et al. (2000)Neurochem Res25, 1439-51.Alternate NamesAIF; Fractionation; Histone H3; Localization; MEK1; MEK2; Vimentin

AIF; Fractionation; Histone H3; Localization; MEK1; MEK2; Vimentin