Cleaved Caspase-3 (Asp175) (5A1E) Rabbit Monoclonal Antibody (SignalFlexTMAlexa Fluor®555 Conjugate)#59916,Cell Signaling Technology (CST),59916

Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb (SignalFlexTM Alexa Fluor®555 Conjugate) detects endogenous levels of the large fragment (17/19 kDa) of activated caspase-3 resulting from cleavage adjacent to Asp175. This antibody does not recognize full-length caspase-3 or other cleaved caspases. Non-specific labeling may be observed by immunofluorescence in specific sub-types of healthy cells in fixed-frozen tissues (e.g. pancreatic alpha-cells). Cytoplasmic background may be observed in human and monkey samples.

Host

Rabbit

Reactivity

Human, Mouse, Rat, Monkey

Conjugate

SignalFlexTMAlexa Fluor®555 Conjugate

Platform ID

BAB818986222

Cell Signaling Technology (CST)

Headquarters

3 Trask Lane Danvers, MA 01923

Cell Signaling Technology (CST)

Contact

Tel: 877-616-2355,978-867-2388
Fax: 877-616-2355

Email:

[email protected]

Product Specifications
Scientific Background
Synonyms

Specifications

NameCleaved Caspase-3 (Asp175) (5A1E) Rabbit Monoclonal Antibody (SignalFlexTMAlexa Fluor®555 Conjugate)#59916
Cat. No.59916
Accession NumberP42574
Gene ID (Entrez)42574
HostRabbit
SensitivityEndogenous
ReactivityHuman, Mouse, Rat, Monkey
ConjugationSignalFlexTMAlexa Fluor®555 Conjugate
ImmunogenIgG
FormulationSignalFlexTM conjugates are produced using highly validated Cell Signaling Technology®primary antibodies and conjugation methods that have been rigorously tested, ensuring high-quality conjugates and lot-to-lot consistency. These conjugates are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. However, they are not tested on specific assays.Optimal dilutions/concentrations should be determined by the end user. When performing flow cytometry, we recommend using an isotype control conjugate at the same concentration as the antibody conjugate.
StorageSupplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C.Do not aliquot the antibody. Protect from light. Do not freeze.
Regulatory StatusResearch Use Only

Scientific Background

Caspase-3 (CPP-32, Apopain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins, such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP) (1). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires the aspartic acid residue at the P1 position (2).Alternate NamesApopain; CASP-3; CASP3; caspase 3; caspase 3, apoptosis-related cysteine peptidase; caspase 3, apoptosis-related cysteine protease; Caspase-3; Caspase-3 subunit p12; Caspase-3 subunit p17; caspase3; CPP-32; CPP32; CPP32B; Cysteine protease CPP32; PARP cleavage protease; procaspase3; Protein Yama; SCA-1; SREBP cleavage activity 1; Yama

Synonyms

Apopain; CASP-3; CASP3; caspase 3; caspase 3, apoptosis-related cysteine peptidase; caspase 3, apoptosis-related cysteine protease; Caspase-3; Caspase-3 subunit p12; Caspase-3 subunit p17; caspase3; CPP-32; CPP32; CPP32B; Cysteine protease CPP32; PARP cleavage protease; procaspase3; Protein Yama; SCA-1; SREBP cleavage activity 1; Yama

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