Cleaved Caspase-9 (Asp330) (D2D4) Rabbit Monoclonal Antibody#7237,Cell Signaling Technology (CST),7237

Cleaved Caspase-9 (Asp330) (D2D4) Rabbit Monoclonal Antibody recognizes endogenous levels of caspase-9 protein only when cleaved at Asp330. Full-length caspase-9 may be weakly detected with some cell lines.

Host

Rabbit

Reactivity

Human

Application

Western Blotting: 1:1000 Immunoprecipitation: 1:100

Platform ID

BAB158579461

Cell Signaling Technology (CST)

Headquarters

3 Trask Lane Danvers, MA 01923

Cell Signaling Technology (CST)

Contact

Tel: 877-616-2355,978-867-2388
Fax: 877-616-2355

Email:

[email protected]

Product Specifications
Scientific Background
Synonyms

Specifications

NameCleaved Caspase-9 (Asp330) (D2D4) Rabbit Monoclonal Antibody#7237
Cat. No.7237
Accession NumberP55211
Gene ID (Entrez)55211
HostRabbit
SensitivityEndogenous
ReactivityHuman
ApplicationWestern Blotting: 1:1000 Immunoprecipitation: 1:100
Molecular Weight37
ImmunogenIgG
FormulationSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
StorageSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Regulatory StatusResearch Use Only

Scientific Background

Caspase-9 (ICE-LAP6, Mch6) is an important member of the cysteine aspartic acid protease (caspase) family (1,2). Upon apoptotic stimulation, cytochrome c released from mitochondria associates with the 47 kDa procaspase-9/Apaf-1. Apaf-1 mediated activation of caspase-9 involves intrinsic proteolytic processing, resulting in cleavage at Asp315 and producing a p35 subunit. Another cleavage occurs at Asp330, producing a p37 subunit that can serve to amplify the apoptotic response (3-6). Cleaved caspase-9 further processes other caspase members, including caspase-3 and caspase-7, to initiate a caspase cascade, which leads to apoptosis (7-10).Duan, H. et al. (1996)J. Biol. Chem.271, 16720-16724.Srinivasula, S. M. et al. (1996)J. Biol. Chem.271, 27099-27106.Liu, X. et al. (1996)Cell86, 147-157.Li, P. et al. (1997)Cell91, 479-489.Zou, H. et al. (1999)J. Biol. Chem.274, 11549-11556.Srinivasula, S.M. et al. (1998)Mol Cell1, 949-57.Deveraux, Q. L. et al. (1998)EMBO J.17, 2215-2223.Slee, E. A. et al. (1999)J. Cell Biol.144, 281-292.Sun, X.M. et al. (1999)J Biol Chem274, 5053-60.MacFarlane, M. et al. (1997)J. Cell Biol.137, 469-479.Alternate NamesAPAF-3; APAF3; apoptotic protease activating factor 3; Apoptotic protease Mch-6; Apoptotic protease-activating factor 3; CASP-9; CASP9; caspase 9; caspase 9, apoptosis-related cysteine peptidase; caspase 9, apoptosis-related cysteine protease; Caspase-9; Caspase-9 subunit p10; Caspase-9 subunit p35; CASPASE-9c; ICE-LAP6; ICE-like apoptotic protease 6; MCH6; PPP1R56; protein phosphatase 1, regulatory subunit 56

Synonyms

APAF-3; APAF3; apoptotic protease activating factor 3; Apoptotic protease Mch-6; Apoptotic protease-activating factor 3; CASP-9; CASP9; caspase 9; caspase 9, apoptosis-related cysteine peptidase; caspase 9, apoptosis-related cysteine protease; Caspase-9; Caspase-9 subunit p10; Caspase-9 subunit p35; CASPASE-9c; ICE-LAP6; ICE-like apoptotic protease 6; MCH6; PPP1R56; protein phosphatase 1, regulatory subunit 56

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