Cyclin Dependent Kinase 1 Phospho-Tyr15 (CDK1 pY15) Antibody,Abbexa,abx000105

CDK1 (pY15) Antibody is a Rabbit Polyclonal antibody against CDK1 (pY15). The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Host

Rabbit

Reactivity

Human, Mouse, Rat

Application

ELISA, WB, IHC, IF/ICC, IP

Conjugate

Unconjugated

Platform ID

BAB680012043

Abbexa

Headquarters

181 Cambridge Science Park Cambridge, CB4 0GJ United Kingdom

Contact

Tel: +44 (0) 1223 755950
Fax: +44 (0) 1223 755951

Product Specifications

Specifications

NameCyclin Dependent Kinase 1 Phospho-Tyr15 (CDK1 pY15) Antibody
Cat. No.abx000105
Accession NumberP06493
Gene ID (Entrez)983
HostRabbit
IsotypeIgG
ReactivityHuman, Mouse, Rat
ConjugationUnconjugated
ApplicationELISA, WB, IHC, IF/ICC, IP
Working DilutionsELISA: 1 µg/ml, WB: 1/500 - 1/1000, IHC-P: 1/50 - 1/200, IF/ICC: 1/50 - 1/200, IP: 0.5 µg - 4 µg antibody per 200 µg - 400 µg extracts of whole cells. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user.
ClonalityPolyclonal
Concentration> 0.2 mg/ml
Molecular WeightCalculated MW: 34 kDa Observed MW: 34 kDa
TargetCDK1
ImmunogenSynthetic peptide corresponding to CDK1 pY15. The exact sequence is proprietary.
PurityPurified by affinity chromatography.
Appearance/FormLiquid
FormulationPBS, pH 7.3, containing 0.05% Proclin-300, 50% glycerol.
StorageAliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
Regulatory StatusResearch Use Only

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