DDDDK tag antibody,Genetex,GTX73143

Host

Rabbit

Reactivity

Species independent

Application

WB ICC/IF IP Dot

Conjugate

Unconjugated

Platform ID

BAB861333472

Genetex

Headquarters

2456 Alton Pkwy Irvine, CA 92606 USA

Contact

Tel: 1-949-553-1900
Fax: 1-949-309-2888

Product Specifications
Scientific Background

Specifications

NameDDDDK tag antibody
Cat. No.GTX73143
HostRabbit
IsotypeIgG
ReactivitySpecies independent
ConjugationUnconjugated
ApplicationWB ICC/IF IP Dot
ClonalityPolyclonal
Concentration~0.8 mg/ml (Please refer to the vial label for the specific concentration.)
ImmunogenFLAG; peptide sequence DYKDDDDK
PurityPurified by affinity chromatography
Appearance/FormLiquid
FormulationPBS, 1% BSA,15mM Sodium azide
StorageStore as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Regulatory StatusResearch Use Only

Scientific Background

Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins.

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