DHCR7 (F7I5B) Rabbit Monoclonal Antibody#62115,Cell Signaling Technology (CST),62115

DHCR7 (F7I5B) Rabbit mAb recognizes endogenous levels of total DHCR7 protein.

Host

Rabbit

Reactivity

Human, Mouse, Rat

Application

Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:400 - 1:1600

Platform ID

BAB286140731

Cell Signaling Technology (CST)

Headquarters

3 Trask Lane Danvers, MA 01923

Cell Signaling Technology (CST)

Contact

Tel: 877-616-2355,978-867-2388
Fax: 877-616-2355

Email:

[email protected]

Product Specifications
Scientific Background
Synonyms

Specifications

NameDHCR7 (F7I5B) Rabbit Monoclonal Antibody#62115
Cat. No.62115
Accession NumberQ9UBM7
Gene ID (Entrez)1717
HostRabbit
SensitivityEndogenous
ReactivityHuman, Mouse, Rat
ApplicationWestern Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:400 - 1:1600
Molecular Weight40
ImmunogenIgG
FormulationSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C.Do not aliquot the antibody.
StorageSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C.Do not aliquot the antibody.
Regulatory StatusResearch Use Only

Scientific Background

7-dehydrocholesterol reductase (DHCR7) catalyzes the conversion of 7-dehydrocholesterol (7-DHC) to cholesterol, the last step of cholesterol biosynthesis. Studies show that DHCR7 expression is downregulated in macrophages upon viral infection. DHCR7 deficiency activates the PI3K-AKT3 pathway. AKT3 phosphorylates interferon regulatory factor 3 (IRF3) at Ser385, leading to IRF3 activation and increased type I interferon (IFN-I) production in macrophages (1). In addition, DHCR7 has proferroptotic activity. Its substrate, 7-DHC, suppresses ferroptosis by inhibiting phospholipid peroxidation. Blocking 7-DHC biosynthesis enhances ferroptosis of cancer cells (2,3).Xiao, J. et al. (2020)Immunity52, 109-122.e6.Freitas, F.P. et al. (2024)Nature626, 401-410.Li, Y. et al. (2024)Nature626, 411-418.Alternate Names7-dehydrocholesterol reductase; 7-DHC reductase; D7SR; delta-7-dehydrocholesterol reductase; Delta7-sterol reductase; DHCR7; Putative sterol reductase SR-2; SLOS; sterol delta-7-reductase; Sterol Delta(7)-reductase; Sterol reductase SR-2

Synonyms

7-dehydrocholesterol reductase; 7-DHC reductase; D7SR; delta-7-dehydrocholesterol reductase; Delta7-sterol reductase; DHCR7; Putative sterol reductase SR-2; SLOS; sterol delta-7-reductase; Sterol Delta(7)-reductase; Sterol reductase SR-2

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