DNAJC9 (F4U7W) Rabbit Monoclonal Antibody#69542,Cell Signaling Technology (CST),69542

DNAJC9 (F4U7W) Rabbit mAb recognizes endogenous levels of total DNAJC9 protein.

Host

Rabbit

Reactivity

Human, Monkey

Application

Western Blotting: 1:1000 Simple WesternTM: 1:10 - 1:50 Immunofluorescence (Immunocytochemistry): 1:200 - 1:800

Platform ID

BAB536565527

Cell Signaling Technology (CST)

Headquarters

3 Trask Lane Danvers, MA 01923

Cell Signaling Technology (CST)

Contact

Tel: 877-616-2355,978-867-2388
Fax: 877-616-2355

Email:

[email protected]

Product Specifications
Scientific Background
Synonyms

Specifications

NameDNAJC9 (F4U7W) Rabbit Monoclonal Antibody#69542
Cat. No.69542
Accession NumberQ8WXX5
Gene ID (Entrez)23234
HostRabbit
SensitivityEndogenous
ReactivityHuman, Monkey
ApplicationWestern Blotting: 1:1000 Simple WesternTM: 1:10 - 1:50 Immunofluorescence (Immunocytochemistry): 1:200 - 1:800
Molecular Weight30
ImmunogenIgG
FormulationSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C.Do not aliquot the antibody.
StorageSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C.Do not aliquot the antibody.
Regulatory StatusResearch Use Only

Scientific Background

DnaJ homolog subfamily C member 9 (DNAJC9) serves a dual role as both a histone chaperone and a heat shock co-chaperone. As a histone chaperone, DNAJC9 forms a co-chaperone complex with MCM2 and histone H3-H4 heterodimers, potentially assisting MCM2 in recognizing these heterodimers and facilitating their assembly into nucleosomes. DNAJC9 interacts with HSP70, activating its ATPase activity (1).Overexpression of CENP-A, a centromeric histone H3 variant, is frequently observed in cancer. Its mislocalization to noncentromeric regions promotes chromosomal instability (CIN), a hallmark of cancer. DNAJC9 plays a crucial role in preventing the mislocalization of centromeric CENP-A, thereby mitigating chromosome instability (2). DNAJC9 expression is a potential prognostic biomarker in basal-like and luminal A breast cancer (3).Hammond, C.M. et al. (2021)Mol Cell81, 2533-2548.e9.Balachandra, V. et al. (2024)EMBO J43, 2166-2197.Incekara, O. and Acun, T. (2023)Mol Biol Rep50, 7275-7282.Alternate NamesDnaJ (Hsp40) homolog, subfamily C, member 9; DnaJ heat shock protein family (Hsp40) member C9; DnaJ homolog subfamily C member 9; DnaJ protein SB73; DNAJC9; DNJC9; HDJC9; JDD1; KIAA0974; SB73

Synonyms

DnaJ (Hsp40) homolog, subfamily C, member 9; DnaJ heat shock protein family (Hsp40) member C9; DnaJ homolog subfamily C member 9; DnaJ protein SB73; DNAJC9; DNJC9; HDJC9; JDD1; KIAA0974; SB73

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