DYKDDDDK Tag (D6W5B) Rabbit Monoclonal Antibody (Binds to same epitope as Sigma-Aldrich Anti-FLAG M2 antibody)#14793,Cell Signaling Technology (CST),14793

DYKDDDDK Tag (D6W5B) Rabbit Monoclonal Antibody (Binds to same epitope as Sigma-Aldrich Anti-FLAG M2 antibody) detects exogenously expressed DYKDDDDK proteins in cells. This antibody recognizes the DYKDDDDK peptide, which is the same epitope recognized by Sigma-Aldrich Anti-FLAG M2 antibody, fused to either the amino-terminus or carboxy-terminus of the target protein.

Host

Rabbit

Reactivity

All Species Expected

Application

Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:400 - 1:1600 Immunofluorescence (Immunocytochemistry): 1:400 - 1:1600 Flow Cytometry (Fixed/Permeabilized): 1:800 - 1:3200 Chromatin IP: 1:50

Platform ID

BAB099596522

Cell Signaling Technology (CST)

Headquarters

3 Trask Lane Danvers, MA 01923

Cell Signaling Technology (CST)

Contact

Tel: 877-616-2355,978-867-2388
Fax: 877-616-2355

Email:

[email protected]

Product Specifications
Scientific Background

Specifications

NameDYKDDDDK Tag (D6W5B) Rabbit Monoclonal Antibody (Binds to same epitope as Sigma-Aldrich Anti-FLAG M2 antibody)#14793
Cat. No.14793
HostRabbit
SensitivityTransfected Only
ReactivityAll Species Expected
ApplicationWestern Blotting: 1:1000 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:400 - 1:1600 Immunofluorescence (Immunocytochemistry): 1:400 - 1:1600 Flow Cytometry (Fixed/Permeabilized): 1:800 - 1:3200 Chromatin IP: 1:50
ImmunogenIgG
FormulationFor optimal ChIP results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 106cells) per IP. This antibody has been validated using SimpleChIP®Enzymatic Chromatin IP Kits.
StorageSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.For a carrier free (BSA and azide free) version of this product see product #70586.
Regulatory StatusResearch Use Only

Scientific Background

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.The DYKDDDDK peptide has been used extensively as a general epitope tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion (1).Brizzard, B.L. et al. (1994)Biotechniques16, 730-5.

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