Delta FosB (D3S8R) Rabbit Monoclonal Antibody#14695,Cell Signaling Technology (CST),14695

The Fos family of nuclear oncogenes includes c-Fos, FosB, Fos-related antigen 1 (FRA1), and Fos-related antigen 2 (FRA2) (1). While most Fos proteins exist as a single isoform, the FosB protein exists as two isoforms: full-length FosB and a shorter form, FosB2 (Delta FosB), which lacks the carboxy-terminal 101 amino acids (1-3). The expression of Fos proteins is rapidly and transiently induced by a variety of extracellular stimuli, including growth factors, cytokines, neurotransmitters, polypeptide hormones, and stress. Fos proteins dimerize with Jun proteins (c-Jun, JunB, and JunD) to form Activator Protein-1 (AP-1), a transcription factor that binds to TRE/AP-1 elements and activates transcription. Fos and Jun proteins contain the leucine-zipper motif that mediates dimerization and an adjacent basic domain that binds to DNA. The various Fos/Jun heterodimers differ in their ability to transactivate AP-1 dependent genes. In addition to increased expression, phosphorylation of Fos proteins by Erk kinases in response to extracellular stimuli may further increase transcriptional activity (4-6). Phosphorylation of c-Fos at Ser32 and Thr232 by Erk5 increases protein stability and nuclear localization (5). Phosphorylation of FRA1 at Ser252 and Ser265 by Erk1/2 increases protein stability and leads to overexpression of FRA1 in cancer cells (6). Following growth factor stimulation, expression of FosB and c-Fos in quiescent fibroblasts is immediate, but very short-lived, with protein levels dissipating after several hours (7). FRA1 and FRA2 expression persists longer, and appreciable levels can be detected in asynchronously growing cells (8). Deregulated expression of c-Fos, FosB, or FRA2 can result in neoplastic cellular transformation; however, Delta FosB lacks the ability to transform cells (2,3).The delta FosB protein is encoded by theFosBgene and is produced by alternative splicing. This shorter isoform lacks a carboxy-terminal FosB region that contains ubiquitination sites and results in more stable delta FosB protein (9). Induced delta FosB accumulates in select brain regions upon chronic drug use (10-12) where it interacts with JunD to form an active, long-lasting AP-1 complex (13). This complex may represent a molecular switch that helps initiate and maintain the addicted state (14,15).Tulchinsky, E. (2000)Histol Histopathol15, 921-8.Dobrazanski, P. et al. (1991)Mol Cell Biol11, 5470-8.Nakabeppu, Y. and Nathans, D. (1991)Cell64, 751-9.Rosenberger, S.F. et al. (1999)J Biol Chem274, 1124-30.Sasaki, T. et al. (2006)Mol Cell24, 63-75.Basbous, J. et al. (2007)Mol Cell Biol27, 3936-50.Kovary, K. and Bravo, R. (1991)Mol Cell Biol11, 2451-9.Kovary, K. and Bravo, R. (1992)Mol Cell Biol12, 5015-23.Carle, T.L. et al. (2007)Eur J Neurosci25, 3009-19.Hope, B.T. et al. (1994)Neuron13, 1235-44.Nye, H.E. et al. (1995)J Pharmacol Exp Ther275, 1671-80.Nye, H.E. and Nestler, E.J. (1996)Mol Pharmacol49, 636-45.Chen, J. et al. (1997)J Neurosci17, 4933-41.Nestler, E.J. et al. (2001)Proc Natl Acad Sci U S A98, 11042-6.McClung, C.A. et al. (2004)Brain Res Mol Brain Res132, 146-54.Alternate Namesactivator protein 1; AP-1; DKFZp686C0818; FBJ murine osteosarcoma viral oncogene homolog B; FOSB; FosB proto-oncogene, AP-1 trancription factor subunit; FosB proto-oncogene, AP-1 transcription factor subunit; G0/G1 switch regulatory protein 3; G0S3; GOS3; GOSB; MGC42291; oncogene FOS-B; Protein FosB

activator protein 1; AP-1; DKFZp686C0818; FBJ murine osteosarcoma viral oncogene homolog B; FOSB; FosB proto-oncogene, AP-1 trancription factor subunit; FosB proto-oncogene, AP-1 transcription factor subunit; G0/G1 switch regulatory protein 3; G0S3; GOS3; GOSB; MGC42291; oncogene FOS-B; Protein FosB