Direct-Blot HRP anti-DYKDDDDK Tag Antibody anti-DYKDDDDK - L5,BioLegend,637312
The L5 clone has been demonstrated to have 2-8 fold better sensitivity in WB than another commonly used antibody clone, M2.
Host
Rat
Reactivity
Epitope tag
Application
WB - Quality tested
Platform ID
BAB556900819
BioLegend
Contact
Tel: 1-858-455-9588
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Product Specifications
Scientific Background
Specifications
NameDirect-Blot HRP anti-DYKDDDDK Tag Antibody anti-DYKDDDDK - L5
Cat. No.637312
HostRat
RRIDAB_2734514 (BioLegend Cat. No. 637312)AB_2566706 (BioLegend Cat. No. 637311)
IsotypeRat IgG2a, λ
ReactivityEpitope tag
ApplicationWB - Quality tested
ClonalityMonoclonal
Clone NumberL5
ConcentrationLot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.)
TargetDYKDDDDK
ImmunogenDYKDDDDK-tagged mouse Langerin
PurityThe antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
FormulationThis antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
StorageUpon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Regulatory StatusResearch Use Only
Scientific Background
The DYKDDDDK tag, commonly referred to as Sigma®'s FLAG® Tag, is often used as a protein modification in order to simplify the labeling and detection of proteins.This unique amino acid sequence allows for specific antibody detection in western blotting, immunoprecipitation, and immunostaining techniques.Due to the short sequence, this modification is not likely to affect the structure or function of the modified proteins.
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