Direct-Blot™ HRP anti-E. coliRNA Sigma 70 Recombinant Antibody, Sigma 70, 2G10.RecM1,BioLegend,666054
This recombinant antibody is derived from the original clone 2G10. We sequenced the complementarity-determining regions (CDRs) from the 2G10 hybridoma and grafted them into a mouse IgG1, κ Fc framework. Clone 2G10 specifically recognizes the major sigma factor across multiple bacterial species but shows no reactivity againstBorrelia burgdorferi. Clone 2G10 is known to target epitope 470-486 in the major sigma factor ofE. coli.
Host
Mouse
Reactivity
E. coli
Application
WB - Quality tested
Platform ID
BAB928201360
BioLegend
Contact
Tel: 1-858-455-9588
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Product Specifications
Scientific Background
Specifications
NameDirect-Blot™ HRP anti-E. coliRNA Sigma 70 Recombinant Antibody, Sigma 70, 2G10.RecM1
Cat. No.666054
HostMouse
IsotypeMouse IgG1, κ
ReactivityE. coli
ApplicationWB - Quality tested
ClonalityRecombinant
Clone Number2G10.RecM1
ConcentrationLot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.)
TargetSigma 70
ImmunogenRecombinant protein
PurityThe antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
FormulationThis antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
StorageUpon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Regulatory StatusResearch Use Only
Scientific Background
Most prokaryotes such as E. coli express a single core RNA polymerase to transcribe RNA from DNA. RNA Sigma 70, also known as rpoD, is the major housekeeping member of the sigma factor subunit family which are required for core RNA polymerase to bind to promoters and initiate transcription. RNA sigma 70 is also required for the unwinding of DNA strands to allow access to RNA polymerase.
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