Direct-Blot HRP anti-His Tag Antibody anti-His tag - J095G46,BioLegend,362614

This antibody was raised against a linear 8X His tag. It reacts with proteins displaying repeats of 6 Histidines (His6) or more in either internal or C and N terminal regions. It is suitable for ICFC.Clone J095G46 has been verified for immunocytochemistry (ICC).

Host

Mouse

Reactivity

Human, Mouse, Rat, All species

Application

WB -Quality tested

Platform ID

BAB369745639

BioLegend

Headquarters

8999 BioLegend Way San Diego, CA 92121 United States

Contact

Tel: 1-858-455-9588
Fax: +49 (4131) 7023913

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Product Specifications
Scientific Background

Specifications

NameDirect-Blot HRP anti-His Tag Antibody anti-His tag - J095G46
Cat. No.362614
HostMouse
RRIDAB_2734398 (BioLegend Cat. No. 362613)AB_2734399 (BioLegend Cat. No. 362614)
IsotypeMouse IgG2a, κ
ReactivityHuman, Mouse, Rat, All species
ApplicationWB -Quality tested
ClonalityMonoclonal
Clone NumberJ095G46
ConcentrationLot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.)
TargetHis Tag
ImmunogenKLH-conjugated His-tag peptide.
PurityThe antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
FormulationThis antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
StorageUpon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Regulatory StatusResearch Use Only

Scientific Background

This antibody was raised against a linear 8XHis tag. It reacts with His-tagged protein with C, N or internal 6XHis tags or longer. It can be used for western blotting to detect linear His repeats (6XHis or more). It can also be used for ICFC and ICC staining of His-tagged proteins.

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