Direct-Blot HRP anti-V5-tag Antibody anti-V5-tag - 7/4,BioLegend,680603

Host

Mouse

Reactivity

Epitope tag

Application

WB - Quality tested

Platform ID

BAB821230202

BioLegend

Headquarters

8999 BioLegend Way San Diego, CA 92121 United States

BioLegend

Contact

Tel: 1-858-455-9588
Fax: +49 (4131) 7023913

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Product Specifications
Scientific Background

Specifications

NameDirect-Blot HRP anti-V5-tag Antibody anti-V5-tag - 7/4
Cat. No.680603
HostMouse
RRIDAB_2734532 (BioLegend Cat. No. 680604)AB_2572008 (BioLegend Cat. No. 680603)
IsotypeMouse IgG2b, κ
ReactivityEpitope tag
ApplicationWB - Quality tested
ClonalityMonoclonal
Clone Number46207
ConcentrationLot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.)
TargetV5-tag
PurityThe antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
FormulationThis antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
StorageUpon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Regulatory StatusResearch Use Only

Scientific Background

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. The V5 tag is a 14 amino acid peptide derived from a small epitope (Pk) on the P and V proteins of simian virus 5 (SV5), a member of the paramyxovirus family. Because of its small size, the addition of a V5 tag sequence (GKPIPNPLLGLDST) to a given gene creates a stable fusion product that does not appear to interfere with the biochemical properties of the protein. Many recombinant proteins have been engineered to express a short sequence derived from the V5 molecule known as V5-tag.

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