Direct-Blot HRP anti-beta-actin Antibody anti-beta-actin - 2F1-1,BioLegend,643807
The binding of this antibody to its target is sensitive to salt concentration. For consistent results, please use TBS/T buffer for Western blotting that contains 0.15 M NaCl as indicated in the BioLegend recommendedprotocol.
Host
Mouse
Reactivity
Human, Mouse, Rat
Application
WB - Quality tested
Platform ID
BAB615195353
BioLegend
Contact
Tel: 1-858-455-9588
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Product Specifications
Scientific Background
Specifications
NameDirect-Blot HRP anti-beta-actin Antibody anti-beta-actin - 2F1-1
Cat. No.643807
HostMouse
RRIDAB_2734515 (BioLegend Cat. No. 643808)AB_2566701 (BioLegend Cat. No. 643807)
IsotypeMouse IgG2b, κ
ReactivityHuman, Mouse, Rat
ApplicationWB - Quality tested
ClonalityMonoclonal
Clone Number2F1-1
ConcentrationLot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.)
Targetbeta-actin
PurityThe antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
FormulationThis antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
StorageUpon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Regulatory StatusResearch Use Only
Scientific Background
β-actin is a ubiquitously expressed and highly conserved 42 kD cytoplasmic protein involved in cell motility. This critical cytoskeletal component can be disrupted by drugs such as cytochalasin. Because β-actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection (such as Western blotting).
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