FAK (D5O7U) Rabbit Monoclonal Antibody (BSA and Azide Free)#60557,Cell Signaling Technology (CST),60557

FAK (D5O7U) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total FAK protein. Immunofluorescence was performed on cells plated on tissue culture grade plastic. We did not detect FAK signal on uncoated glass slides.

Host

Rabbit

Reactivity

Human

Platform ID

BAB288939387

Cell Signaling Technology (CST)

Headquarters

3 Trask Lane Danvers, MA 01923

Cell Signaling Technology (CST)

Contact

Tel: 877-616-2355,978-867-2388
Fax: 877-616-2355

Email:

[email protected]

Product Specifications
Scientific Background
Synonyms

Specifications

NameFAK (D5O7U) Rabbit Monoclonal Antibody (BSA and Azide Free)#60557
Cat. No.60557
Accession NumberQ05397
Gene ID (Entrez)05397, 5747
HostRabbit
SensitivityEndogenous
ReactivityHuman
Molecular Weight125
ImmunogenIgG
FormulationThis product is the carrier-free version of product #71433. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN, or CUT&Tag assays. If you require a carrier-free formulation for chromatin profiling, pleasecontact us. Optimal dilutions/concentrations should be determined by the end user.BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.
StorageStore at –20°C.This product will freeze at –20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles.A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.
Regulatory StatusResearch Use Only

Scientific Background

Focal adhesion kinase (FAK) is a widely expressed cytoplasmic protein tyrosine kinase involved in integrin-mediated signal transduction. It plays an important role in the control of several biological processes, including cell spreading, migration, and survival (1). Activation of FAK by integrin clustering leads to autophosphorylation at Tyr397, which is a binding site for the Src family kinases PI3K and PLCγ (2-5). Recruitment of Src family kinases results in the phosphorylation of Tyr407, Tyr576, and Tyr577 in the catalytic domain, and Tyr871 and Tyr925 in the carboxy-terminal region of FAK (6,7).Parsons, J.T. et al. (2000)Oncogene19, 5606-13.Schaller, M.D. et al. (1994)Mol Cell Biol14, 1680-8.Cobb, B.S. et al. (1994)Mol Cell Biol14, 147-55.Chen, H.C. et al. (1996)J Biol Chem271, 26329-34.Zhang, X. et al. (1999)Proc Natl Acad Sci U S A96, 9021-6.Calalb, M.B. et al. (1995)Mol Cell Biol15, 954-63.Schlaepfer, D.D. et al. (1994)Nature372, 786-791.Alternate NamesFADK; FADK 1; FAK; FAK-related non-kinase polypeptide; FAK1; Focal adhesion kinase 1; Focal adhesion kinase-related nonkinase; FRNK; p125FAK; pp125FAK; PPP1R71; Protein phosphatase 1 regulatory subunit 71; protein tyrosine kinase 2; Protein-tyrosine kinase 2; PTK2; PTK2 protein tyrosine kinase 2

Synonyms

FADK; FADK 1; FAK; FAK-related non-kinase polypeptide; FAK1; Focal adhesion kinase 1; Focal adhesion kinase-related nonkinase; FRNK; p125FAK; pp125FAK; PPP1R71; Protein phosphatase 1 regulatory subunit 71; protein tyrosine kinase 2; Protein-tyrosine kinase 2; PTK2; PTK2 protein tyrosine kinase 2

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