FITC anti-human IFN-gamma Antibody anti-IFN-gamma - 4S.B3,BioLegend,502506
ELISA or ELISPOT Detection5:The biotinylated 4S.B3 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified NIB42 antibody (Cat. No. 502402/502404) or purified MD-1 antibody (Cat. No. 507502/507513) as the capture antibody.Flow Cytometry3,4,6-8:The fluorochrome-labeled 4S.B3 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IFN-γ -producing cells within mixed cell populations.Additional reported applications (for the relevant formats) include:neutralization1,2, Western blotting, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated tissue sections, and immunocytochemistry. The 4S.B3 antibody can neutralize the bioactivity of natural or recombinant IFN-γ.Note:For testing human IFN-γ in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 430101 to 430106) are specially developed and recommended.
Host
Mouse
Reactivity
Human
Application
ICFC - Quality tested
Platform ID
BAB812034618
BioLegend
Contact
Tel: 1-858-455-9588
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Product Specifications
Scientific Background
Specifications
NameFITC anti-human IFN-gamma Antibody anti-IFN-gamma - 4S.B3
Cat. No.502506
HostMouse
RRIDAB_315230 (BioLegend Cat. No. 502505)AB_315232 (BioLegend Cat. No. 502507)AB_315231 (BioLegend Cat. No. 502506)
IsotypeMouse IgG1, κ
ReactivityHuman
ApplicationICFC - Quality tested
ClonalityMonoclonal
Clone Number4S.B3
Concentrationµg sizes: 0.5 mg/mLtest sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in ourCertificate of Analysisonline tool.)
TargetIFN-gamma
ImmunogenPartially purified, native human IFN-γ
PurityThe antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Formulationtest sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.
Regulatory StatusResearch Use Only
Scientific Background
Interferon-γ is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells.
Category Paths
- Products>Primary Antibodies>Monoclonal Antibodies
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