FITC anti-mouse CD8a Antibody anti-CD8a - 5H10-1,BioLegend,100803

Additional reported applications (for the relevant formats) include: partial inhibition of T-cell responses to IL-2 and cytotoxic induction of splenic T cells1, and immunohistochemistry2of acetone-fixed frozen sections. The 5H10-1 antibody competes with 53-6.7 antibody (Cat. No. 100702) for binding to thymocytes1.Clone 5H10-1 is not recommended for immunohistochemistry of formalin-fixed paraffin sections.

Host

Rat

Reactivity

Mouse

Application

FC -Quality tested

Platform ID

BAB067856822

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Contact

Tel: 1-858-455-9588
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Product Specifications
Scientific Background

Specifications

NameFITC anti-mouse CD8a Antibody anti-CD8a - 5H10-1
Cat. No.100803
HostRat
RRIDAB_312764 (BioLegend Cat. No. 100803)AB_312765 (BioLegend Cat. No. 100804)
IsotypeRat (SD) IgG2b, λ
ReactivityMouse
ApplicationFC -Quality tested
ClonalityMonoclonal
Clone Number5H10-1
Concentration0.5 mg/ml
TargetCD8alpha
ImmunogenConcanavalin A-stimulated BALB/c splenic T cells
PurityThe antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.
Regulatory StatusResearch Use Only

Scientific Background

CD8, also known as Lyt-2, Ly-2, or T8, consists of disulfide-linked α and β chains that form the α(CD8a)/β(CD8b) heterodimer and α/α homodimer. CD8a is a 34kD protein that belongs to the immunoglobulin family. The CD8 α/β heterodimer is expressed on the surface of most thymocytes and a subset of mature TCR α/β T cells. CD8 expression on mature T cells is non-overlapping with CD4. The CD8 α/α homodimer is expressed on a subset of γ/δ TCR-bearing T cells, NK cells, intestinal intraepithelial lymphocytes, and lymphoid dendritic cells. CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. CD8 promotes T cell activation through its association with the TCR complex and protein tyrosine kinase lck.

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