FITC anti-mouse CD90.2 (Thy1.2) Antibody anti-CD90.2 - 30-H12,BioLegend,105305

Additional reported applications (for the relevant formats) include:in vivoandin vitrodepletion1,2,7, costimulation of CD3/TCR-mediated signal transduction3,4, and immunohistochemical staining5of acetone-fixed frozen sections. The 30-H12 antibody does not react with Thy-1.1 alloantigen of the AKR/J and PL strains. To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 (Cat. No. 101301 & 101302) is recommended prior to immunofluorescent staining. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 105351 & 105352).

Host

Rat

Reactivity

Mouse

Application

FC - Quality tested

Platform ID

BAB992681659

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Product Specifications
Scientific Background

Specifications

NameFITC anti-mouse CD90.2 (Thy1.2) Antibody anti-CD90.2 - 30-H12
Cat. No.105305
HostRat
RRIDAB_313176 (BioLegend Cat. No. 105305)AB_313177 (BioLegend Cat. No. 105306)
IsotypeRat IgG2b, κ
ReactivityMouse
ApplicationFC - Quality tested
ClonalityMonoclonal
Clone Number30-H12
Concentration0.5 mg/ml
TargetCD90.2
ImmunogenMouse thymus or spleen
PurityThe antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.
Regulatory StatusResearch Use Only

Scientific Background

CD90.2 is a 25-35 kD immunoglobulin superfamily member also known as Thy1.2. It is expressed on hematopoietic stem cells and neurons, all thymocytes, and peripheral T cells in Thy1.2 bearing mouse strains (Balb/c, CBA/J, C3H/He, C57BL/-, DBA, NZB/-). CD90.2 is a glycosylphosphatidylinositol (GPI)-anchored membrane glycoprotein involved in signal transduction. CD90.2 is involved in costimulation of lymphocyte proliferation and induction of hematopoietic stem cells differentiation. CD90.2 has been shown to interact with CD45. The 30-H12 antibody has been reported to induce Ca2+flux in thymocytes and, in combination with antibody against the CD3/TCR complex, promote thymocyte apoptosis and inhibit CD3-mediated proliferative responses of mature T lymphocytes.

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