Firefly Luciferase (E9W9V) Rabbit Monoclonal Antibody#25039,Cell Signaling Technology (CST),25039

Firefly Luciferase (E9W9V) Rabbit Monoclonal Antibody recognizes exogenously expressed proteins containing firefly luciferase (P. pyralis). This antibody may also recognize a non-specific band of unknown origin at 70 kDa in rat cell lines.

Host

Rabbit

Reactivity

All Species Expected

Application

Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:50 - 1:200

Platform ID

BAB789589075

Cell Signaling Technology (CST)

Headquarters

3 Trask Lane Danvers, MA 01923

Cell Signaling Technology (CST)

Contact

Tel: 877-616-2355,978-867-2388
Fax: 877-616-2355

Email:

[email protected]

Product Specifications
Scientific Background
Synonyms

Specifications

NameFirefly Luciferase (E9W9V) Rabbit Monoclonal Antibody#25039
Cat. No.25039
Accession NumberP08659, 116160
Gene ID (Entrez)08659, 116160065
HostRabbit
SensitivityTransfected Only
ReactivityAll Species Expected
ApplicationWestern Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:50 - 1:200
Molecular Weight62
ImmunogenIgG
FormulationSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C.Do not aliquot the antibody.
StorageSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C.Do not aliquot the antibody.
Regulatory StatusResearch Use Only

Scientific Background

Luciferases are a group of enzymes that emit visible light in the presence of oxygen and luciferin. Firefly luciferase (FLuc) is a 62 kDa oxygenase isolated from the firefly,ÂP. pyralis, that produces a yellow-green light with a peak emission wavelength of 550-570 nm (1). FLuc is widely used as a bioluminescent reporter, emitting light through an ATP-dependent reaction involving oxygen, D-luciferin, and a metal cation, typically magnesium (Mg2+) (2). FLuc is often genetically engineered into luciferase-containing plasmids as a reporter that can be measured by detection of firefly luciferase (P. pyralis) protein or its enzymatic activity (2,3).de Wet, J.R. et al. (1985)Proc Natl Acad Sci USA82, 7870-3.Thorne, N. et al. (2010)Chem Biol17, 646-57.de Wet, J.R. et al. (1987)Mol Cell Biol7, 725-37.Alternate Namesluciferin 4-monooxygenase

Synonyms

luciferin 4-monooxygenase

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