G4S Linker (E7O2V) Rabbit Monoclonal Antibody (Alexa Fluor®647 Conjugate)#69782,Cell Signaling Technology (CST),69782

G4S Linker (E7O2V) Rabbit Monoclonal Antibody (Alexa Fluor®647 Conjugate) recognizes exogenously expressed levels of scFv-based CARs containing a G4S linker.

Host

Rabbit

Reactivity

All Species Expected

Application

Immunofluorescence (Immunocytochemistry): 1:50 Flow Cytometry (Live): 1:50

Platform ID

BAB695822840

Cell Signaling Technology (CST)

Headquarters

3 Trask Lane Danvers, MA 01923

Cell Signaling Technology (CST)

Contact

Tel: 877-616-2355,978-867-2388
Fax: 877-616-2355

Email:

[email protected]

Product Specifications
Scientific Background

Specifications

NameG4S Linker (E7O2V) Rabbit Monoclonal Antibody (Alexa Fluor®647 Conjugate)#69782
Cat. No.69782
HostRabbit
SensitivityTransfected Only
ReactivityAll Species Expected
ApplicationImmunofluorescence (Immunocytochemistry): 1:50 Flow Cytometry (Live): 1:50
ImmunogenIgG
FormulationThis Cell Signaling Technology antibody is conjugated to Alexa Fluor®647 fluorescent dye under optimal conditions and tested in-house for direct flow cytometric analysis in human cells. This antibody conjugate is expected to exhibit the same reactivity as the unconjugated G4S Linker (E7O2V) Rabbit mAb #71645, which is expected to react with cell surface-expressed CARs of varying specificity containing a G4S linker within the scFv of the extracellular domain.
StorageSupplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C.Do not aliquot the antibody. Protect from light. Do not freeze.
Regulatory StatusResearch Use Only

Scientific Background

The poly-Glycine-Serine (G4S) linker is a type of flexible, unstructured synthetic peptide linker sequence often leveraged to connect the variable heavy (VH) domain and variable light (VL) domain of single-chain variable fragments (scFvs) and chimeric antigen receptors (CARs) that utilize an extracellular domain scFv for target antigen recognition. The linker itself consists of a core pentapeptide sequence, Gly-Gly-Gly-Gly-Ser, that is repeated and commonly found as either a 15-mer (G4S)3or20-mer(G4S)4within scFv-based CARs and scFv fragments. The linker sequence length plays a role in controlling scFv stability and the noncovalent association between the VH and VL domains (1,2).Huston, J.S. et al. (1988)Proc Natl Acad Sci USA85, 5879-83.Chen, X. et al. (2013)Adv Drug Deliv Rev65, 1357-69.

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