G4S Linker (E7O2V) Rabbit Monoclonal Antibody (Biotinylated)#17621,Cell Signaling Technology (CST),17621

The poly-Glycine-Serine (G4S) linker is a type of flexible, unstructured synthetic peptide linker sequence often leveraged to connect the variable heavy (VH) domain and variable light (VL) domain of single-chain variable fragments (scFvs) and chimeric antigen receptors (CARs) that utilize an extracellular domain scFv for target antigen recognition. The linker itself consists of a core pentapeptide sequence, Gly-Gly-Gly-Gly-Ser, that is repeated and commonly found as either a 15-mer (G4S)3or20-mer(G4S)4within scFv-based CARs and scFv fragments. The linker sequence length plays a role in controlling scFv stability and the noncovalent association between the VH and VL domains (1,2).Huston, J.S. et al. (1988)Proc Natl Acad Sci USA85, 5879-83.Chen, X. et al. (2013)Adv Drug Deliv Rev65, 1357-69.