GFP antibody,Genetex,GTX30738

Host

Rabbit

Reactivity

Species independent

Application

WB ICC/IF IHC-P FCM IP ELISA ChIP assay Gel supershift assays IM IHC (Free Floating)

Conjugate

Unconjugated

Platform ID

BAB435981417

Genetex

Headquarters

2456 Alton Pkwy Irvine, CA 92606 USA

Contact

Tel: 1-949-553-1900
Fax: 1-949-309-2888

Product Specifications
Scientific Background

Specifications

NameGFP antibody
Cat. No.GTX30738
HostRabbit
IsotypeIgG
ReactivitySpecies independent
ConjugationUnconjugated
ApplicationWB ICC/IF IHC-P FCM IP ELISA ChIP assay Gel supershift assays IM IHC (Free Floating)
ClonalityPolyclonal
ImmunogenThe GFP was isolated directly from the jellyfish Aequorea victoria.
PurityUnpurified
Appearance/FormLiquid
FormulationSerum,0.01% Thimerosal
StorageStore as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Regulatory StatusResearch Use Only

Scientific Background

A highly versatile antibody that gives a stronger signal than other anti-GFP antibodies available. On Western blot the antibody detects the GFP fraction from cell extracts expressing recombinant GFP fusion proteins and has also been shown to be useful on mouse sections fixed with formalin (Mesaeli et al. 1999). In Immunocytochemistry, the antibody gives a very good signal on recombinant YES-GFP chimeras expressed in COS cells (McCabe et al. 1999 and figure below). It is routinely used in Immunoprecipitation (IP) and IP-Western protocols and has been used successfully in HRP Immunohistochemistry at 1:200 on whole-mount mouse embryos.

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