Glutamate Transporter Antibody Explorer Kit,Alomone Labs,AK-440_KIT

The transport of Glu into the cells by the EAAT transporters is coupled to the Na + and K + electrochemical gradient as a driving force. Hence, the uptake of Glu is dependent on the co-transport of three Na + and one H + ions, and the counter transport of one K + ion. In addition, to the well documented Glu uptake, the EAAT transporters show a Glu-independent Cl - conductance. The physiological significance of the Cl - current through the EAATs is currently unknown. 1,2 EAAT1 as well as EAAT2 , is expressed predominantly in glia cells (hence its original name: glial glutamate transporter or GLAST), while EAAT3 , EAAT4 and EAAT5 are mostly expressed in neurons. Three vesicular glutamate transporters (VGLUTs) have been cloned: BNPI/VGLUT1 (a brain-specific sodium dependent inorganic phosphate (Pi) transporter), and its homologs DNPI/VGLUT2 (differentiation-associated sodium-dependent Pi transporter) and VGLUT3 3 . These transporters mediate glutamate uptake inside presynaptic vesicles and are anatomical and functional markers of glutamatergic excitatory transmission 4 .