Glutaminase-1/GLS1 (E4T9Q) Rabbit Monoclonal Antibody (BSA and Azide Free)#84021,Cell Signaling Technology (CST),84021

Glutaminase-1/GLS1 (E4T9Q) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total glutaminase-1/GLS1 protein. This antibody does not cross-react with glutaminase-2/GLS2 protein.

Host

Rabbit

Reactivity

Human

Platform ID

BAB416600609

Cell Signaling Technology (CST)

Headquarters

3 Trask Lane Danvers, MA 01923

Cell Signaling Technology (CST)

Contact

Tel: 877-616-2355,978-867-2388
Fax: 877-616-2355

Email:

[email protected]

Product Specifications
Scientific Background
Synonyms

Specifications

NameGlutaminase-1/GLS1 (E4T9Q) Rabbit Monoclonal Antibody (BSA and Azide Free)#84021
Cat. No.84021
Accession NumberO94925
Gene ID (Entrez)94925, 2744
HostRabbit
SensitivityEndogenous
ReactivityHuman
Molecular Weight55-65
ImmunogenIgG
FormulationThis product is the carrier free version of product #49363. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, pleasecontact us. Optimal dilutions/concentrations should be determined by the end user.BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.
StorageStore at -20°C.This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles.A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.
Regulatory StatusResearch Use Only

Scientific Background

Glutaminase catalyzes the conversion of glutamine to glutamate, the first and rate-limiting step of glutaminolysis (1). Both kidney-type glutaminase (GLS1) and liver-type glutaminase (GLS2) are found in mammals (2). GLS1-mediated glutathione synthesis plays an essential role in redox homeostasis and contributes to increased survival of postimplantation bone cells preconditioned to the hypoxic and ischemic environment in the bone defect site (3). In addition,KEAP1–NRF2-mutant LUAD (KRAS-mutant lung adenocarcinoma) tumors are dependent on increased glutaminolysis (1). Furthermore, recent studies showed higher glutaminolysis and glucose production from glutamine in human primary hepatocytes withGLS2gain-of-function missense mutations (4). These findings suggest GLS1 and GLS2 as potential targets in the therapy of bone regeneration and in the treatments of diseases such as cancer and hyperglycemia, respectively (1,3,4).Romero, R. et al. (2017)Nat Med23, 1362-1368.Aledo, J.C. et al. (2000)Mamm Genome11, 1107-10.Stegen, S. et al. (2016)Cell Metab23, 265-79.Miller, R.A. et al. (2018)Nat Med24, 518-524.Alternate NamesAAD20; CASGID; DKFZp686O15119; EIEE71; FLJ10358; GAC; GAM; GDPAG; GLS; GLS1; GLSK; glutaminase; glutaminase C; Glutaminase kidney isoform, mitochondrial; Glutaminase kidney isoform, mitochondrial 65 kDa chain; Glutaminase kidney isoform, mitochondrial 68 kDa chain; glutaminase, phosphate-activated; K-glutaminase; KGA; KIAA0838; L-glutamine amidohydrolase

Synonyms

AAD20; CASGID; DKFZp686O15119; EIEE71; FLJ10358; GAC; GAM; GDPAG; GLS; GLS1; GLSK; glutaminase; glutaminase C; Glutaminase kidney isoform, mitochondrial; Glutaminase kidney isoform, mitochondrial 65 kDa chain; Glutaminase kidney isoform, mitochondrial 68 kDa chain; glutaminase, phosphate-activated; K-glutaminase; KGA; KIAA0838; L-glutamine amidohydrolase

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