GoInVivo Purified anti-human CD274 B7-H1, PD-L1 Antibody anti-CD274 - 29E.2A3,BioLegend,329726

Clone 29E.2A3 is reported to recognize an epitope on PD-L1 within the PD-L1-CD80 binding region5. Additional reported applications (for the relevant formats) include: blocking1-3and immunohistochemical staining of acetone-fixed frozen sections1. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 329715, 329716, 329745 - 329748).It has been observed that clone 29E.2A3 is able to bind to Alexa Fluor® 700 antibody conjugates during multi-color immunofluorescent staining. This interaction can be resolved by sequentially staining with the 29E.2A3 antibody first and then followed by the Alexa Fluor® 700 conjugate of interest.Clone 29E.2A3 does not work in Western blot applications7.

Host

Mouse

Reactivity

Human

Application

FC - Quality testedIHC, Block - Reported in the literature, not verified in house

Platform ID

BAB362271757

BioLegend

Headquarters

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Contact

Tel: 1-858-455-9588
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Product Specifications
Scientific Background

Specifications

NameGoInVivo Purified anti-human CD274 B7-H1, PD-L1 Antibody anti-CD274 - 29E.2A3
Cat. No.329726
HostMouse
RRIDAB_2566287 (BioLegend Cat. No. 329728)AB_2566288 (BioLegend Cat. No. 329729)AB_2566289 (BioLegend Cat. No. 329730)AB_2566080 (BioLegend Cat. No. 329725)AB_2566081 (BioLegend Cat. No. 329726)AB_2566082 (BioLegend Cat. No. 329727)
IsotypeMouse IgG2b, κ
ReactivityHuman
ApplicationFC - Quality testedIHC, Block - Reported in the literature, not verified in house
ClonalityMonoclonal
Clone Number29E.2A3
ConcentrationThe antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL.
TargetCD274
ImmunogenFull length human PD-L1
PurityThe GoInVivo™ antibody was purified by affinity chromatography.
Formulation0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C. This GoInVivo™ solution contains no preservative; handle under aseptic conditions.
Regulatory StatusResearch Use Only

Scientific Background

CD274, also known as PD-L1 and B7-H1, is type I transmembrane glycoprotein that serves as a ligand for CD279 (PD-1). This interaction is believed to regulate the balance between the stimulatory and inhibitory signals needed for responses to microbes and maintenance of self-tolerance. CD274 is involved in the costimulation of T cell proliferation and IL-10 and IFN-γ production in an IL-2-dependent and CD279-independent manner. Conflicting data has shown that CD274 can inhibit T cell proliferation and cytokine production, and alternatively, enhance T cell activation. Other studies suggest that CD274 may signal bidirectionally, raising interesting implications for its expression in a wide variety of cell types, including T and B cells, antigen-presenting cells, and nonhematopoietic cells.

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