GoInVivo Purified anti-mouse CD152 Antibody anti-CD152 - 9H10,BioLegend,106209

Additional reported applications (for the relevant formats) include:in vivoandin vitroblocking1-4of CTLA-4 action by soluble antibody and promotion ofin vitroCTLA-4 signalling by immobilized antibody1. To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 (Cat. No. 101301/101302), is recommended prior to immunofluorescent staining. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-Syrian hamster IgG (Cat. No. 405601) second step, followed by SAv-PE (Cat. No. 405204)).

Host

Syrian Hamster

Reactivity

Mouse

Application

ELISA -Quality testedBlock, Costim, FC -Reported in the literature, not verified in house

Platform ID

BAB138834001

BioLegend

Headquarters

8999 BioLegend Way San Diego, CA 92121 United States

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Contact

Tel: 1-858-455-9588
Fax: +49 (4131) 7023913

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Product Specifications
Scientific Background

Specifications

NameGoInVivo Purified anti-mouse CD152 Antibody anti-CD152 - 9H10
Cat. No.106209
HostSyrian Hamster
RRIDAB_2616629 (BioLegend Cat. No. 106205)AB_2616630 (BioLegend Cat. No. 106206)AB_2616631 (BioLegend Cat. No. 106207)AB_2616632 (BioLegend Cat. No. 106208)AB_2616633 (BioLegend Cat. No. 106209)AB_2616634 (BioLegend Cat. No. 106210)
IsotypeSyrian Hamster IgG
ReactivityMouse
ApplicationELISA -Quality testedBlock, Costim, FC -Reported in the literature, not verified in house
ClonalityMonoclonal
Clone Number9H10
ConcentrationThe antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL.
TargetCD152
ImmunogenMouse CTLA-4-human IgG1 fusion protein
PurityThe GoInVivo™ antibody was purified by affinity chromatography.
Formulation0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C. This GoInVivo™ solution contains no preservative; handle under aseptic conditions.
Regulatory StatusResearch Use Only

Scientific Background

CD152 is a 33 kD member of the immunoglobulin superfamily also known as CTLA-4 or Ly-56. It is expressed on activated T and B lymphocytes. CD152 is similar to CD28 in amino acid sequence, structure, and genomic organization and these two receptors share common B7 family counter-receptors (B7-1, B7-2). Whereas CD28 delivers a costimulatory signal in T cell activation, CTLA-4 negatively regulates cell-mediated immune responses. CD152 is thought to play a role in the induction and maintenance of immunological tolerance, as well as the development of protective immunity and thymocyte regulation. The 9H10 antibody can enhance T cell co-stimulation by blocking CTLA-4 interactions with the B7 co-receptors, favoring CD28 interactions.

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