GoInVivo Purified anti-mouse CD274 B7-H1, PD-L1 Antibody anti-CD274 - 10F.9G2,BioLegend,124327

Additional reported applications (for the relevant formats) include: immunofluorescence4, blocking6,7,8,9, and immunohistochemistry of acetone-fixed frozen sections4, 11. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 124303). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 124318) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Host

Rat

Reactivity

Mouse

Application

FC - Quality testedBlock - Reported in the literature, not verified in house

Platform ID

BAB725564879

BioLegend

Headquarters

8999 BioLegend Way San Diego, CA 92121 United States

BioLegend

Contact

Tel: 1-858-455-9588
Fax: +49 (4131) 7023913

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Product Specifications
Scientific Background

Specifications

NameGoInVivo Purified anti-mouse CD274 B7-H1, PD-L1 Antibody anti-CD274 - 10F.9G2
Cat. No.124327
HostRat
RRIDAB_2566281 (BioLegend Cat. No. 124328)AB_2566282 (BioLegend Cat. No. 124329)AB_2566283 (BioLegend Cat. No. 124330)AB_2566092 (BioLegend Cat. No. 124325)AB_2566093 (BioLegend Cat. No. 124326)AB_2566094 (BioLegend Cat. No. 124327)
IsotypeRat IgG2b, κ
ReactivityMouse
ApplicationFC - Quality testedBlock - Reported in the literature, not verified in house
ClonalityMonoclonal
Clone Number10F.9G2
ConcentrationThe antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL.
TargetCD274
PurityThe GoInVivo™ antibody was purified by affinity chromatography.
Formulation0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C. This GoInVivo™ solution contains no preservative; handle under aseptic conditions.
Regulatory StatusResearch Use Only

Scientific Background

CD274, also known as B7-H1 or programmed death ligand 1 (PD-L1), is a 40 kD type I transmembrane protein and a member of the B7 family within the immunoglobulin receptor superfamily. It is expressed on T cells, B cells, NK cells, dendritic cells, IFN-γ activated endothelial cells, and monocytes. B7-H1 is one of the ligands of PD-1. The interaction of B7-H1 with PD-1 plays an important role in the inhibition of T cell responses. Other studies have shown that B7-H1 is able to costimulate T cell growth and cytokine production. CD274 is involved in costimulation essential for T cell proliferation and production of IL-10 and IFN-γ, in an IL-2-dependent and a PD-1-independent manner. Its interaction with PD-1 inhibits T cell proliferation and cytokine production.

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