GoInVivo Purified anti-mouse CD279 PD-1 Antibody anti-CD279 - RMP1-14,BioLegend,114114

Additional reported applications (for relevant formats of this clone) include: immunohistochemical staining of acetone-fixed frozen sections1, Western blotting2,6, inhibition of macrophages (but not B cells and dendritic cells) costimulated T cell proliferation3,5, and blocking the binding of PD-1 to B7-H1 or B7-DC3,4,8.

Host

Rat

Reactivity

Mouse

Application

FC - Quality testedIHC, WB, FA - Reported in the literature, not verified in house

Platform ID

BAB086236731

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Contact

Tel: 1-858-455-9588
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Product Specifications
Scientific Background

Specifications

NameGoInVivo Purified anti-mouse CD279 PD-1 Antibody anti-CD279 - RMP1-14
Cat. No.114114
HostRat
RRIDAB_2566278 (BioLegend Cat. No. 114114)AB_2566279 (BioLegend Cat. No. 114115)AB_2566280 (BioLegend Cat. No. 114116)AB_2566089 (BioLegend Cat. No. 114111)AB_2566090 (BioLegend Cat. No. 114112)AB_2566091 (BioLegend Cat. No. 114113)
IsotypeRat IgG2a, κ
ReactivityMouse
ApplicationFC - Quality testedIHC, WB, FA - Reported in the literature, not verified in house
ClonalityMonoclonal
Clone NumberRMP1-14
ConcentrationThe antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL.
TargetCD279
ImmunogenMouse PD-1 transfected BHK cells
PurityThe GoInVivo™ antibody was purified by affinity chromatography.
Formulation0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C. This GoInVivo™ solution contains no preservative; handle under aseptic conditions.
Regulatory StatusResearch Use Only

Scientific Background

CD279 is a 50-55 kD immunoglobulin superfamily member, also known as programmed death-1 (PD-1). PD-1 is expressed on a subset of CD4-CD8-thymocytes, and on activated T and B cells. PD-1 is thought to be involved in lymphocyte clonal selection and peripheral tolerance. The PD-1 ligands, PD-L1 (also known as B7-H1) and PD-L2 (B7-DC), are members of the B7 immunoglobulin superfamily. The RMP1-14 antibody has been reported to block the binding of PD-1 to its ligands (B7-H1 and B7-DC) and to inhibit T cell proliferation and cytokine production costimulated by macrophages (but not by dendritic cells and B cells).

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