GoInVivo™ Purified anti-mouse TNF-α Antibody, TNF-α, MP6-XT22,BioLegend,506351

ELISA Capture: The purified MP6-XT22 antibody is useful as the capture antibody in a sandwich ELISA when used in conjunction with the biotinylated Poly5160 antibody (Cat. No. 516003) as the detection antibody and recombinant mouse TNF-α (Cat. No. 575209) as the standard.Flow Cytometry6,11,12:The fluorochrome-labeled MP6-XT22 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify TNF-a-producing cells within mixed cell populations.Neutralization1,5,10,16,17:The MP6-XT22 antibody can neutralize the bioactivity of natural or recombinant TNF-α. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of mouse TNF-α bioactivityin vivoandin vitro(Cat. No. 506310). Forin vivostudies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 506332) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).Additional reported applications (for the relevant formats) include: Western blotting, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections7-9in vivodetection5, immunofluorescence, and immunocytochemistry.Note: For testing mouse TNF-α in serum, plasma or supernatant, BioLegend's ELISA Max™ Sets (Cat. No. 430901) are specially developed and recommended.

Host

Rat

Reactivity

Mouse

Application

ELISA, ICFC - Quality testedCyTOF®, ICC - VerifiedIHC-F, WB, Neut - Reported in the literature, not verified in house

Platform ID

BAB507013512

BioLegend

Headquarters

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BioLegend

Contact

Tel: 1-858-455-9588
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Product Specifications
Scientific Background

Specifications

NameGoInVivo™ Purified anti-mouse TNF-α Antibody, TNF-α, MP6-XT22
Cat. No.506351
HostRat
RRIDAB_2888813 (BioLegend Cat. No. 506351)AB_2888813 (BioLegend Cat. No. 506352)AB_2888813 (BioLegend Cat. No. 506353)AB_2888813 (BioLegend Cat. No. 506354)AB_2888813 (BioLegend Cat. No. 506355)AB_2888813 (BioLegend Cat. No. 506356)
IsotypeRat IgG1, κ
ReactivityMouse
ApplicationELISA, ICFC - Quality testedCyTOF®, ICC - VerifiedIHC-F, WB, Neut - Reported in the literature, not verified in house
ClonalityMonoclonal
Clone NumberMP6-XT22
ConcentrationThe antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL.
TargetTNF-alpha
ImmunogenE. coli-expressed, recombinant mouse TNF-α
PurityThe GoInVivo™ antibody was purified by affinity chromatography.
Formulation0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C. This GoInVivo™ solution contains no preservative; handle under aseptic conditions.
Regulatory StatusResearch Use Only

Scientific Background

TNF-α is secreted by macrophages, monocytes, neutrophils, T-cells, and NK-cells. Many transformed cell lines also secrete TNF-α. Monomeric mouse TNF-α is a 156 amino acid protein (N-glycosylated) with a reported molecular weight of 17.5 kD. TNF-α forms multimeric complexes; stable trimers are most common in solution. A 26 kD membrane form of TNF-α has also been described. TNF-α binding to surface receptors elicits a wide array of biologic activities including: cytolysis and cytostasis of many tumor cell linesin vitro, hemorrhagic necrosis of tumorsin vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils.

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