GoInVivo™ Purified anti-mouse/rat IL-1β Antibody, IL-1β, B122,BioLegend,503507

ELISA or ELISPOT Capture3:The purified B122 antibody is useful as the capture antibody in a mouse IL-1ß sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated Poly5158 antibody (Cat. No. 515801) as the detecting antibody. The LEAF™ purified antibody is suggested for ELISPOT capture.Certain formats of clone B122 have been reported forin vitroneutralization of IL-1ß bioactivity1,in vivocytokine depletion5, immunohistochemical staining of frozen sections6, Western blotting7, and the study of endotoxin shock8.

Host

Armenian Hamster

Reactivity

Mouse, Rat

Application

ELISA Capture - Quality testedELISPOT Capture, Depletion, IHC-F, IP, Neut, WB - Reported in the literature, not verified in house

Platform ID

BAB456637704

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Contact

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Product Specifications
Scientific Background

Specifications

NameGoInVivo™ Purified anti-mouse/rat IL-1β Antibody, IL-1β, B122
Cat. No.503507
HostArmenian Hamster
RRIDAB_2888752 (BioLegend Cat. No. 503507)AB_2888752 (BioLegend Cat. No. 503508)AB_2888752 (BioLegend Cat. No. 503509)AB_2888752 (BioLegend Cat. No. 503510)AB_2888752 (BioLegend Cat. No. 503511)AB_2888752 (BioLegend Cat. No. 503512)
IsotypeArmenian Hamster IgG
ReactivityMouse, Rat
ApplicationELISA Capture - Quality testedELISPOT Capture, Depletion, IHC-F, IP, Neut, WB - Reported in the literature, not verified in house
ClonalityMonoclonal
Clone NumberB122
ConcentrationThe antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL.
TargetIL-1beta
ImmunogenE. coli-expressed, recombinant mouse IL-1β
PurityThe GoInVivo™ antibody was purified by affinity chromatography.
Formulation0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
StorageThe antibody solution should be stored undiluted between 2°C and 8°C. This GoInVivo™ solution contains no preservative; handle under aseptic conditions.
Regulatory StatusResearch Use Only

Scientific Background

IL-1 refers to two proteins, IL-1α and IL-1β which are the products of distinct genes, but which are recognized by the same cell surface receptors. IL-1β is a potent immuno-modulator which mediates a wide range of immune and inflammatory responses including the activation of B and T cells. The B122 antibody reacts with the precursor and mature secreted forms of mouse IL-1β. This antibody also recognizes rat IL-1β. The B122 antibody can neutralize the bioactivity of natural or recombinant IL-1β.

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