Goat Anti-Rabbit IgG, Fc Fragment Antibody (FITC Conjugate)#86426,Cell Signaling Technology (CST),86426

Goat Anti-Rabbit IgG, Fc Fragment Antibody (FITC Conjugate) reacts with the Fc portion of rabbit IgG. This antibody conjugate does not react with the Fab portion of rabbit IgG or human serum proteins.

Reactivity

BackgroundThis product is optimized for use as a secondary antibody in immunofluorescent applications. Fluorescent anti-species IgG conjugates are ideal for immunofluorescence. Cell Signaling Technology applies strict quality control procedures to ensure that each conjugate provides optimal specificity and fluorescence.

Platform ID

BAB390701782

Cell Signaling Technology (CST)

Headquarters

3 Trask Lane Danvers, MA 01923

Cell Signaling Technology (CST)

Contact

Tel: 877-616-2355,978-867-2388
Fax: 877-616-2355

Email:

[email protected]

Product Specifications
Scientific Background

Specifications

NameGoat Anti-Rabbit IgG, Fc Fragment Antibody (FITC Conjugate)#86426
Cat. No.86426
ReactivityBackgroundThis product is optimized for use as a secondary antibody in immunofluorescent applications. Fluorescent anti-species IgG conjugates are ideal for immunofluorescence. Cell Signaling Technology applies strict quality control procedures to ensure that each conjugate provides optimal specificity and fluorescence.
FormulationThe optimal dilution of the anti-species antibody should be determined for each primary antibody by titration. However, a final dilution of 1:400 – 1:1600 should yield acceptable results for immunofluorescent assays.When using rabbit Fc-directed secondaries, a higher concentration of primary antibody may be required. The optimal dilution of each primary antibody should be determined by titration.FITC has relatively low photostability. Usage of an antifade reagent is recommended to preserve signal intensity.
StorageSupplied lyophilized in a formulation of 0.02 M potassium phosphate, 0.15 M sodium chloride (pH 7.2), 10 mg/mL BSA, and 0.01% azide. Store lyophilized product for up to 12 months at 4C. Reconstitute lyophilized material with 1.0 mL dH2O and centrifuge until clear ​(if needed​). Once reconstituted, the undiluted liquid product is stable for six weeks at 4ºC. ​For extended storage, aliquot and freeze at -20C or below​ to avoid multiple freeze/thaw cycles.​ Dilute to a working solution immediately prior to use.
Regulatory StatusResearch Use Only

Scientific Background

This product is optimized for use as a secondary antibody in immunofluorescent applications. Fluorescent anti-species IgG conjugates are ideal for immunofluorescence. Cell Signaling Technology applies strict quality control procedures to ensure that each conjugate provides optimal specificity and fluorescence.

Category Paths

Request a product

Please provide the required information below so that we can quickly source your products.