HA-Tag (C29F4) Rabbit Monoclonal Antibody (Alexa Fluor®555 Conjugate)#55420,Cell Signaling Technology (CST),55420

HA-Tag (C29F4) Rabbit Monoclonal Antibody (Alexa Fluor®555 Conjugate) detects exogenously expressed proteins containing the HA epitope tag.

Host

Rabbit

Reactivity

All Species Expected

Application

Immunofluorescence (Immunocytochemistry): 1:200 - 1:800 Flow Cytometry (Fixed/Permeabilized): 1:50

Platform ID

BAB509078727

Cell Signaling Technology (CST)

Headquarters

3 Trask Lane Danvers, MA 01923

Contact

Tel: 877-616-2355,978-867-2388
Fax: 877-616-2355

Product Specifications
Scientific Background

Specifications

NameHA-Tag (C29F4) Rabbit Monoclonal Antibody (Alexa Fluor®555 Conjugate)#55420
Cat. No.55420
HostRabbit
SensitivityTransfected Only
ReactivityAll Species Expected
ApplicationImmunofluorescence (Immunocytochemistry): 1:200 - 1:800 Flow Cytometry (Fixed/Permeabilized): 1:50
ImmunogenIgG
FormulationSupplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C.Do not aliquot the antibody. Protect from light. Do not freeze.
StorageSupplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C.Do not aliquot the antibody. Protect from light. Do not freeze.
Regulatory StatusResearch Use Only

Scientific Background

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.The HA tag is derived from an epitope of the influenza hemagglutinin protein which has been used extensively as a general epitope tag in expression vectors (1).Field, J. et al. (1988)Mol Cell Biol8, 2159-65.

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