HRP Anti-Histone H3 antibody - Nuclear Loading Control,Abcam,AB21054

What is this antibody validated in? HRP Anti-Histone H3 antibody - Nuclear Loading Control (ab21054) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Drosophila melanogaster, Human, Mouse, Saccharomyces cerevisiae samples. What is the molecular weight of Histone H3? Anti-Histone H3 - Nuclear Loading Control HRP (ab21054) is often used as a loading control in Western blot. It specifically detects a band for Histone H3 (UniProt: P68431) at a molecular weight of 15.4kDa. Trusted by the scientific community Anti-Histone H3 - Nuclear Loading Control HRP (ab21054) was first used in a scientific publication in 2007 and has been cited over 20 times in peer-reviewed journals.

Host

Rabbit

Reactivity

Mouse, Human, Saccharomyces cerevisiae, Drosophila melanogaster

Application

WB, IHC-P

Conjugate

HRP

Platform ID

BAB902812173

Abcam

Headquarters

Discovery Drive Cambridge Biomedical Campus Cambridge CB2 0AX UK

Contact

Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215

Product Specifications
Scientific Background

Specifications

NameHRP Anti-Histone H3 antibody - Nuclear Loading Control
Cat. No.AB21054
HostRabbit
IsotypeIgG
ReactivityMouse, Human, Saccharomyces cerevisiae, Drosophila melanogaster
ConjugationHRP
ApplicationWB, IHC-P
ClonalityPolyclonal
Concentration0.5 mg/mL Batch dependent concentration
ImmunogenThe exact immunogen used to generate this antibody is proprietary information.
PurityAffinity purification Immunogen
Appearance/FormLiquid
ShippingBlue Ice
FormulationpH: 7.4 Preservative: 0.1% Proclin 300 Solution Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Storage-20°C
Regulatory StatusResearch Use Only

Scientific Background

Target data The protein expressed by gene H3C1 is a core component of the nucleosome, which is essential for wrapping and compacting DNA into chromatin. This process restricts DNA accessibility to cellular machineries that use DNA as a template. Consequently, histones are central to transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is regulated through a complex set of post-translational modifications of histones, known as the histone code, and through nucleosome remodeling. This supplementary information is collated from multiple sources and compiled automatically. See full target information H3C1

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