HRP Anti-beta Actin antibody [AC-15] - Loading Control,Abcam,AB49900

HRP Anti-beta Actin antibody [AC-15] (ab49900) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in WB in african green monkey, cow, dog, hamster, human, mouse, rat samples. HRP Anti-beta Actin antibody [AC-15] (ab49900) has been cited over 457 times in peer reviewed journals and is trusted by the scientific community. HRP Anti-beta Actin antibody [AC-15] (ab49900) has 10 independent reviews from customers. HRP Anti-beta Actin antibody [AC-15] (ab49900) specifically detects beta Actin (UniProt ID: P60709; Molecular weight: 42kDa) and is sold in a 50 uL selling size.

Host

Mouse

Reactivity

Hamster, Mouse, Rat, Cow, Dog, Human, African green monkey

Application

WB

Conjugate

HRP

Platform ID

BAB025675699

Abcam

Headquarters

Discovery Drive Cambridge Biomedical Campus Cambridge CB2 0AX UK

Abcam

Contact

Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215

Email:

[email protected]

Product Specifications
Scientific Background

Specifications

NameHRP Anti-beta Actin antibody [AC-15] - Loading Control
Cat. No.AB49900
HostMouse
IsotypeIgG1
ReactivityHamster, Mouse, Rat, Cow, Dog, Human, African green monkey
ConjugationHRP
ApplicationWB
ClonalityMonoclonal
Clone NumberAC-15
Concentration3.6 mg/mL Batch dependent concentration
ImmunogenSynthetic Peptide within Human ACTB aa 1-50 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.
PurityAffinity purification Protein A
Appearance/FormLiquid
ShippingBlue Ice
FormulationpH: 7.4 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS
Storage+4°C
Regulatory StatusResearch Use Only

Scientific Background

Target data Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells (PubMed : 25255767, PubMed : 29581253). Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction (PubMed : 29581253). In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA (PubMed : 29925947). Plays a role in the assembly of the gamma-tubulin ring complex (gTuRC), which regulates the minus-end nucleation of alpha-beta tubulin heterodimers that grow into microtubule protafilaments (PubMed : 39321809, PubMed : 38609661). Part of the ACTR1A/ACTB filament around which the dynactin complex is built (By similarity). The dynactin multiprotein complex activates the molecular motor dynein for ultra-processive transport along microtubules (By similarity). See full target information ACTB

Category Paths

Request a product

Please provide the required information below so that we can quickly source your products.