HRP anti-Neurofilament H (NF-H), Nonphosphorylated Antibody anti-Neurofilament H - SMI 32,BioLegend,801707

Additional reported applications (for the relevant formats) include Western blotting6, immunohistochemistry4,5, immunocytochemistry1,2,3, 7, array tomography8.Cross-reactivity to monkey tissue has been Reported in the literature, not verified in house4.This antibody reacts with a nonphosphorylated epitope in neurofilament H of most mammalian species. The reaction is masked when the epitope is phosphorylated. The staining of isolated neurofilament preparations is greatly intensified upon dephosphorylation. Immunocytochemically, SMI 32 visualizes neuronal cell bodies, dendrites, and some thick axons in the central and peripheral nervous systems. However, thin axons are not revealed. Other cells and tissues are unreactive. The antibody distinguishes three subdivisions of the macaque precentral motor cortex. The greater size of the left versus the right superior temporal lobe was found to be due to increased axonal myelination and not due to increased number of glial cells or SMI 32-enumerated neurons, suggesting that the specialization for language in the left temporal lobe is related to increased speed of signal transmission. In cultures of murine cortex, SMI 32 labels a neuronal population with enhanced vulnerability to kainate toxicity most of which are GABAergic and reveal kainate-activated Ca2+uptake.

Host

Mouse

Reactivity

Human, Mouse, Rat

Application

IHC-P -Quality testedWB -Verified

Platform ID

BAB327454684

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Product Specifications
Scientific Background

Specifications

NameHRP anti-Neurofilament H (NF-H), Nonphosphorylated Antibody anti-Neurofilament H - SMI 32
Cat. No.801707
HostMouse
RRIDAB_2721607 (BioLegend Cat. No. 801707)AB_2721608 (BioLegend Cat. No. 801708)
IsotypeMouse IgG1
ReactivityHuman, Mouse, Rat
ApplicationIHC-P -Quality testedWB -Verified
ClonalityMonoclonal
Clone NumberSMI 32
Concentration0.5 mg/ml
TargetNeurofilament H
PurityThe antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
FormulationThis antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
StorageUpon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Regulatory StatusResearch Use Only

Scientific Background

Neurofilaments (NF) are approximately 10 nanometer intermediate filaments found in neurons. They are a major component of the neuronal cytoskeleton, and function primarily to provide structural support for the axon and to regulate the axon diameter. There are three major NF subunits, and the names given to these subunits are based upon the apparent molecular mass of the mammalian subunits on SDS-PAGE. The light or lowest NF (NF-L) runs at 68-70 kD. The medium or middle NF (NF-M) runs at about 145-160 kD, and the heavy or highest NF (NF-H) runs at 200-220 kD. However, the actual molecular weight of these proteins is considerably lower due to the highly charged C-terminal regions of the molecules. The level of NF gene expression correlates with the axonal diameter, which controls how fast electrical signals travel down the axon. Mutant mice with NF abnormalities have phenotypes resembling amyotrophic lateral sclerosis. NF immunostaining is common in diagnostic neuropathology. It is useful for differentiating neurons (positive for NF) from the glia (negative for NF).

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