Helios (D4Z6D) Rabbit Monoclonal Antibody#36426,Cell Signaling Technology (CST),36426

Helios (D4Z6D) Rabbit Monoclonal Antibody recognizes endogenous levels of total Helios protein.

Host

Rabbit

Reactivity

Human

Application

Western Blotting: 1:1000 Immunoprecipitation: 1:200 Flow Cytometry (Fixed/Permeabilized): 1:100 Chromatin IP: 1:50 CUT&RUN: 1:50 CUT&Tag: 1:50

Platform ID

BAB230056400

Cell Signaling Technology (CST)

Headquarters

3 Trask Lane Danvers, MA 01923

Cell Signaling Technology (CST)

Contact

Tel: 877-616-2355,978-867-2388
Fax: 877-616-2355

Email:

[email protected]

Product Specifications
Scientific Background
Synonyms

Specifications

NameHelios (D4Z6D) Rabbit Monoclonal Antibody#36426
Cat. No.36426
Accession NumberQ9UKS7
Gene ID (Entrez)22807
HostRabbit
SensitivityEndogenous
ReactivityHuman
ApplicationWestern Blotting: 1:1000 Immunoprecipitation: 1:200 Flow Cytometry (Fixed/Permeabilized): 1:100 Chromatin IP: 1:50 CUT&RUN: 1:50 CUT&Tag: 1:50
Molecular Weight58, 63-70, 85
ImmunogenIgG
FormulationFor optimal ChIP results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 106cells) per IP. This antibody has been validated using SimpleChIP®Enzymatic Chromatin IP Kits.The CUT&RUN dilution was determined using CUT&RUN Assay Kit #86652.
StorageSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.For a carrier free (BSA and azide free) version of this product see product #54887.
Regulatory StatusResearch Use Only

Scientific Background

Helios (Ikaros family zinc finger 2, IKZF2) is an Ikaros family transcription factor composed of several zinc fingers that mediate DNA binding and homodimerization or heterodimerization with other Ikaros family proteins (1,2). In the hematopoietic system, Helios expression is restricted to T cells and early hematopoietic progenitors (1,2). In regulatory T cells, expression of Helios contributes to an anergic phenotype by binding to the IL-2 promoter and suppressing IL-2 transcription (3). In addition, alteration of the corresponding Helios geneIKZF2is one hallmark of low-hypodiploid acute lymphoblastic leukemia (4).Hahm, K. et al. (1998)Genes Dev12, 782-96.Kelley, C.M. et al. (1998)Curr Biol8, 508-15.Baine, I. et al. (2013)J Immunol190, 1008-16.Holmfeldt, L. et al. (2013)Nat Genet45, 242-52.Alternate NamesANF1A2; HELIOS; IKAROS family zinc finger 2; IKAROS family zinc finger 2 (Helios); Ikaros family zinc finger protein 2; IKZF2; MGC34330; zinc finger DNA binding protein Helios; Zinc finger protein Helios; zinc finger protein, subfamily 1A, 2 (Helios); ZNF1A2; ZNFN1A2

Synonyms

ANF1A2; HELIOS; IKAROS family zinc finger 2; IKAROS family zinc finger 2 (Helios); Ikaros family zinc finger protein 2; IKZF2; MGC34330; zinc finger DNA binding protein Helios; Zinc finger protein Helios; zinc finger protein, subfamily 1A, 2 (Helios); ZNF1A2; ZNFN1A2

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