Horseradish Peroxidase antibody,Genetex,GTX22116

Host

Sheep

Reactivity

Horseradish

Application

WB ELISA

Conjugate

Unconjugated

Platform ID

BAB112680354

Genetex

Headquarters

2456 Alton Pkwy Irvine, CA 92606 USA

Contact

Tel: 1-949-553-1900
Fax: 1-949-309-2888

Product Specifications
Scientific Background

Specifications

NameHorseradish Peroxidase antibody
Cat. No.GTX22116
HostSheep
IsotypeIgG
ReactivityHorseradish
ConjugationUnconjugated
ApplicationWB ELISA
ClonalityPolyclonal
Concentration10 mg/ml (Please refer to the vial label for the specific concentration.)
ImmunogenHorseradish peroxidase
PurityIgG fractionThis product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
Appearance/FormLiquid
Formulation20mM Potassium Phosphate, 150mM NaCl,No preservatives
StorageStore as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Regulatory StatusResearch Use Only

Scientific Background

Horseradish Peroxidase (HRP) is an enzyme commonly used as an indicator for chemical reactions which produce peroxide. The enzyme is routinely conjugated to antibodies for use in enzyme-based immunoassay systems. HRP functions in the removal of H2O2 (hydrogen peroxide), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue. HRP binds 1 heme B (iron-protoporphyrin IX) group and 2 calcium ions per subunit. Subunits are monomeric.

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