Human Acrosin (ACR) ELISA Kit,Abbkine,KTE60884
• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Platform ID
BAB581242692
Abbkine
Contact
Tel: +1-404-854-0155
Fax:
Email:
Specifications
Scientific Background
Acrosinis the major proteinase present in the acrosome of mature spermatozoa. It is a typical serine proteinase with trypsin-like specificity. It is stored in the acrosome in its precursor form, proacrosin. The active enzyme functions in the lysis of the zona pellucida, thus facilitating penetration of the sperm through the innermost glycoprotein layers of the ovum. In many species, it is shown that biosynthesis of acrosin is confined to the haploid phase of spermatogenesis. By indirect immunofluorescent techniques, Florke-Gerloff et al. demonstrated that in man (pro)acrosin first appears in the haploid spermatids. Adham et al. isolated a full-length cDNA clone for human proacrosin. The deduced amino acid sequence of human proacrosin in the proline-rich domain is different from the corresponding sequence of boar proacrosin.
Synonyms
ACR; Preproacrosin; proacrosin
Category Paths
- Products>ELISA & Assay Kits>ELISA Kits
Please provide the required information below so that we can quickly source your products.