Human IgG isotype control (AP),Genetex,GTX35066

Host

Human

Application

WB ELISA

Conjugate

Alkaline Phosphatase (AP)

Platform ID

BAB517953316

Genetex

Headquarters

2456 Alton Pkwy Irvine, CA 92606 USA

Contact

Tel: 1-949-553-1900
Fax: 1-949-309-2888

Product Specifications
Scientific Background
Synonyms

Specifications

NameHuman IgG isotype control (AP)
Cat. No.GTX35066
HostHuman
IsotypeIgG
ConjugationAlkaline Phosphatase (AP)
ApplicationWB ELISA
ClonalityPolyclonal
Appearance/FormLiquid
Formulation50mM Tris, 1mM MgCl₂, 50% Glycerol,0.1% Sodium azide
StorageStore as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.
Regulatory StatusResearch Use Only

Scientific Background

Inclusion of an appropriate isotype control is essential for the proper interpretation of experimental results. Because isotype control antibodies have no relevant specificity, they enable the researcher to distinguish non-specific "background" binding from antigen-specific antibody binding. Non-specific binding of primary antibodies may occur due to either binding of antibody to Fc receptors or non-specific interactions with cellular proteins, lipids, or carbohydrates. Additionally, autofluorescence or endogenous enzyme activity in target cells may give a false positive that could be misinterpreted as antibody binding. These factors vary by target cell type and the isotype of the primary antibody. Therefore, the primary antibody used should be matched to an isotype control of identical species and isotype (including both heavy and light chains).

Synonyms

Human IgG isotype control , Human isotype control for IgG , Polyclonal Human IgG isotype control , Human IgG negative control , Negative control for Human IgG , Isotype control for Human IgG , Human negative control for IgG , Isotype control to Human IgG

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