Human Meiotic recombination protein SPO11 (SPO11) ELISA Kit,Abbkine,KTE60333
• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Platform ID
BAB451782334
Abbkine
Contact
Tel: +1-404-854-0155
Fax:
Email:
Specifications
Scientific Background
SPO11 is similar in sequence and conserved features to the yeast meiotic recombination protein. The encoded protein belongs to the TOP6A protein family. Several transcript variants encoding different isoforms have been found for this gene, but the full-length nature of only two of them have been described. The human and the mouse SPO11 proteins share 82% amino acid identity, but only 20 to 30% with other eukaryotic homologs such as Drosophila and C. elegans. Both the mouse and human SPO11 exons 2 and 8 are subject to alternative splicing. Northern blot analysis showed that mouse Spo11 is expressed in testis and thymus as a 1.8-kb mRNA, whereas human SPO11 is expressed in testis as a 2.0-kb transcript. RT-PCR revealed that human SPO11 is also expressed in prostate, fetal testis, thymus, and some carcinoma cell lines.
Synonyms
SPO11; CT35; MGC39953; SPO11 meiotic protein covalently bound to DSB-like; SPO11; meiotic protein covalently bound to DSB-like; cancer/testis antigen 35; meiotic recombination protein SPO11
Category Paths
- Products>ELISA & Assay Kits>ELISA Kits
Please provide the required information below so that we can quickly source your products.