Human N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase (NAGPA) ELISA Kit,Abbkine,KTE61386
• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Platform ID
BAB199658354
Abbkine
Contact
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Specifications
Scientific Background
Hydrolases are transported to lysosomes after binding to mannose 6-phosphate receptors in the trans-Golgi network. NAGPA encodes the enzyme that catalyzes the second step in the formation of the mannose 6-phosphate recognition marker on lysosomal hydrolases. Commonly known as 'uncovering enzyme' or UCE, this enzyme removes N-acetyl-D-glucosamine (GlcNAc) residues from GlcNAc-alpha-P-mannose moieties and thereby produces the recognition marker. The encoded preproprotein is proteolytically processed by furin to generate the mature enzyme, a homotetramer of two disulfide-linked homodimers. Mutations in NAGPA are associated with developmental stuttering in human patients
Synonyms
NAGPA; APAA; UCE; alpha-N-acetylglucosaminyl phosphodiesterase; lysosomal alpha-N-acetylglucosaminidase; mannose 6-phosphate-uncovering enzyme; phosphodiester alpha-GlcNAcase
Category Paths
- Products>ELISA & Assay Kits>ELISA Kits
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