Human Pepsin (PG) ELISA Kit,Abbkine,KTE61240
• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Platform ID
BAB579743412
Abbkine
Contact
Tel: +1-404-854-0155
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Specifications
Scientific Background
Pepsin is an enzyme that whose precursor form (pepsinogen) is released by the chief cells in the stomach and that degrades food proteins into peptides. Pepsin was discovered in 1836 by Theodor Schwann who also coined this enzyme's name from the Greek word pepsis, meaning digestion (peptein: to digest). It was the first animal enzyme to be discovered, and, in 1929, it became one of the first enzymes to be crystallized, by John H. Northrop. Pepsin is a digestive protease.Pepsin functions best in acidic environments and is often found in an acidic environment, particularly those with a pH of 1.5 to 2. Pepsin denatures if the pH is more than 5.0.Pepsins should be stored at very cold temperatures (between −20°C and −80°C) to prevent autolysis (self-cleavage). Autolysis may also be prevented by storage of pepsins at pH 11 or by using modified pepsins (e.g., by reductive methylation). When the pH is adjusted back to 6.0 activity returns.
Synonyms
PG
Category Paths
- Products>ELISA & Assay Kits>ELISA Kits
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