Human Plasmin-antiplasmin complex (PAP) ELISA Kit,Abbkine,KTE61307
• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Platform ID
BAB387102131
Abbkine
Contact
Tel: +1-404-854-0155
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Specifications
Scientific Background
α2-Antiplasmin is the principal plasma inhibitor of plasmin, with which it rapidly and irreversibly forms a complex. The simple technique of crossed immunoelectrophoresis against antiserum to α2-antiplasmin has been applied to detect -antiplasmin complex in plasma. The presence of this complex is demonstrated in patients in whom overactive fibrinolysis was the only or major contributor to severe haemorrhagic disorders arising spontaneously. When streptokinase-activated, urokinase-activated, or spontaneously activated human plasmin is mixed with increasing amounts of human antiplasmin, caseinolytic effect decreases much more rapidly than fibrinolytic effect. At concentrations of antiplasmin at which caseinolytic effect completely disappears, considerable fibrinolytic activity persists.
Synonyms
PAP
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