Human Rap guanine nucleotide exchange factor 4 (RAPGEF4) ELISA Kit,Abbkine,KTE60978
• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Platform ID
BAB201257586
Abbkine
Contact
Tel: +1-404-854-0155
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Specifications
Scientific Background
Sequence analysis predicted that the 1,011-amino acid cAMP-GEFII protein contains an N-terminal cAMP-binding domain and a C-terminal GEF domain. Expression of cAMP-GEFI and cAMP-GEFII activated RAP1A after forskolin and 3-isobutyl-1-methylxanthine stimulation, independent of the protein kinase A pathway. cAMP-GEFI and cAMP-GEFII expression did not activate or only slightly activated other RAS superfamily members after stimulation. Northern blot analysis detected expression of a 4.4-kb cAMP-GEFII transcript that was most prominent in brain and adrenal gland. Within the brain, expression was strongest in cortex, occipital pole, frontal lobe, temporal lobe, amygdala, putamen, hippocampus, and cerebellum.
Synonyms
RAPGEF4; CAMP-GEFII; CGEF2; EPAC2; Nbla00496; RAP guanine-nucleotide-exchange factor (GEF) 4; cAMP-regulated guanine nucleotide exchange factor; exchange protein directly activated by cAMP 2; putative protei
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