Human Sestrin-1 (SESN1) ELISA Kit,Abbkine,KTE60687
• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Platform ID
BAB954630966
Abbkine
Contact
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Specifications
Scientific Background
Only the smaller transcript was transiently induced by p53 or by genotoxic agents in a p53-dependent manner in a colon carcinoma cell line. The deduced proteins, which are 99% identical to the mouse proteins, contain 551, 491, and 426 amino acids, respectively. RT-PCR analysis detected induction of T2 in response to p53 or genotoxic stress; however, T1 was not induced and T3 was only weakly induced. Western blot analysis showed induction of a predominant 55-kD T2 nuclear protein rather than the 68-kD T1 protein or the 48-kD T3 protein. Sequence analysis predicted, and EMSA and reporter analysis confirmed, that PA26 has a p53-binding site within intron 2. Serum starvation experiments suggested that PA26 may be a member of the growth arrest- and DNA damage-inducible, or GADD, gene family.
Synonyms
SESN1; MGC138241; MGC142129; PA26; RP11-787I22.1; SEST1; p53 regulated PA26 nuclear protein
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- Products>ELISA & Assay Kits>ELISA Kits
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